首页|Identification of key enzymes in lignocellulose biosynthesis from dynamic observations in maize stalks

Identification of key enzymes in lignocellulose biosynthesis from dynamic observations in maize stalks

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Maize stalk lignin and cellulose contents are linked to lodging resistance,disease resistance,feed quality and ethanol conversion efficiency.After the six-leaf stage of maize(V6),these constituents are biosynthe-sized and accumulated under the control of related enzymes and genes.However,the key enzymes,crit-ical MYB transcription factors,and their dynamic alterations pattern under natural field circumstances are still unknown.Hence,we selected five cultivars with significant differences in lignocellulose content and lodging resistance as testing materials,performed field experiments for two years,and investigated the dynamics of lignin and cellulose content,related enzyme concentrations,and gene expression levels in the 3rd and 5th internodes above the ground after V6.The results showed that lignin and cellulose con-tent increased after V6,stabilizing during the silking stage.This study identified COMT(caffeic acid 3-O-methyltransferase),TAL(tyrosine ammonia-lyase)and PAL(phenylalanine ammonia-lyase)as the key enzymes of lignin biosynthesis,while ZmCOMT,ZmCesA10 and ZmCesA8 were identified as essential genes.ZmMYB8,ZmMYB31 and ZmMYB39 were involved in regulating the expression of genes related to lignin synthesis,with ZmMYB31 potentially acting as a key negative regulator,while ZmMYB39 and ZmMYB8 acting as positive regulators.The study also found that around 14 d after V6 was a critical stage for regulating lignocellulose synthesis in the 3rd to 5th basal internode.This provides a theoretical foun-dation for developing regulatory techniques and breeding new cultivars to enhance lodging and disease resistance as well as the utility of maize stalks.

LigninCelluloseBiosynthesisKey enzymeMaize stalk

Xiaoxu Zhan、Fanlei Kong、Qinlin Liu、Tianqiong Lan、Fan Liu、Xinglong Wang、Xia Du、Xiang Chen、Jichao Yuan

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Sichuan Agricultural University,Key Laboratory of Crop Physiology,Ecology,and Cultivation Southwest,Ministry of Agriculture,Chengdu 611130,Sichuan,China

College of Agriculture,Sichuan Agricultural University,Chengdu 611130,Sichuan,China

Photobiology Research Center,Institute of Urban Agriculture,Chinese Academy of Agricultural Sciences,Chengdu 610000,Sichuan,China

2024

10.1016/j.cj.2024.09.017

作物学报(英文版)

作物学报(英文版)

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ISSN：
年,卷(期)：2024.12(6)