Development of the A&GBE base editor for efficiently generating abundant genetic variation in rice

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外文摘要：We have developed a dual base editor,rA&GBE,by fusing adenine and glycosylase base editors.It can induce up to eight types of mutations in T0-generation rice,including single-base conversion,simultane-ous multiple-base conversions,and InDels,using a single guide RNA.A-to-G and C-to-G/T conversions occur simultaneously on the same DNA strand.The rA&GBE system may prove useful for crop improve-ment and in planta direct evolution.

外文关键词：

CRISPRA&GBEGenetic variationRice

作者：

Wenhui Zhang、Yaqi Xu、Jingxuan Cui、Yingshuang Xue、Lijie Zhang、Wenjuan Wu、Chong Wang、Hui Zhang

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作者单位：

Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development,Shanghai Key Laboratory of Plant Molecular Sciences,College of Life Sciences,Shanghai Normal University,Shanghai 200234,China

出版年：

2024

DOI：

10.1016/j.cj.2024.08.009

作物学报(英文版)

CSTPCD

ISSN：

年,卷(期)：2024.12(6)