The chromosome flaking of root-tip on germinate seed in wild Medicago sativa L.can be made clearly,which were pretreated by the mixture of 0.15％ colchicine and 0.002mol/L 8-hydroxyquinoline for 3h, and then,enzymolysissed by 1％ cellulase + 1％ pectinase for 1.5h at 25℃.The result of karyotype analysis shows that the total of chromosome in wild Medicago sativa L.is 32 with a pair of satellites.The karyotype of such specie is 2B type and the karyotype formulation is 2n = 32 = 30m(2SAT) + 2sm, the length construction is 2n = 2L + 18M2 + 8M1 +4S.
NETL
NSTL
维普
万方数据
