Pollen Characteristic and Embryonic Development of Tensei Apple
The pollen characteristics and embryonic development of Fuji(2x)apple and its bud mutation Tensei (4x),were observed by using Scanning Electron Microscope ( SEM) and Laser Scanning Confocal Microscope ( LSCM ). The results showed that the rate of the abnormal pollens was 10. 27% , which was lager than that of diploids. The rate of pollen germination of Tensei was 62. 42% , which was lower than that of diploids. The pollens of both culti-vars were 3-colporate,and the main shape was oblong and two polars of pollen were wide and flat,polars were triangle. But the shape of Tensei was less than Fuji,or the full extent was lower than Fuji. The pollen surface of Tensei was strips ornamentation,with a few of bifurcations and perforations. The pollen surface of Tensei was slightly rough,which of Fuji was smooth. The equatorial axis length and polar axis length of tetraploid were longer than diploid,but P/E value of tetraploid was less than diploid,which was significant difference. The embryo sac of Tensei and Fuji is che-nopodiad-type ,and there was abnormal embryo sac between them. The embryo sac of Tensei was abnormal in varying degrees, including polar nucleaus was in abnormal position, abnormal number of polar nuclei, abnormal structure of egg apparatus and the stasis of embryo development. There were only few incomplete embryo sacs in Fuji.
NETL
NSTL
万方数据
维普
