Origin and Evolution of Cultivated Rice (O.sativa L.) in China Based on Gene Diversity of Chloroplast Genome
To study the origin and evolution of Asian cultivated rice ( Oryza sativa L. ) ,98 accessions of Asian cultivated rice and 125 accessions of common wild rice {Oryza rufipogon) were selected for sequencing analysis. Through sequencing the atpA gene,rpsl6 intron,trnP-rpl33 inter spacer,trnG-trnfM inter spacer and trnT-trnL inter spacer in chloroplast genome in rice, the haplotype network and phylogenetic tree were made with bio-information software. The results showed that common wild rice had more Indels and SNPs compared with the Asian cultivated rice. Both the haplotype network and phylogenetic tree divided all test materials into three groups. Group I consisted by accessions from the Japonica subspecies and the common wild rice, Group II included mainly accessions of Indi-ca subspecies, while Group III were as the accessions of the common wild rice. Importantly, Group II and Group HI had a close relationship, which indicated that the Japonica and Indica Subspecies were originated from Japonica-like and Indica-like common wild rice separately, supporting the Double Domestication Hypotheses of Asia cultivated rice. All the materials of common wild rice with a close relationship to Asian cultivated rice were identified to be collected from South China, implicating South China might be the origin center of Asian cultivated rice.