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基于叶绿体基因多样性的中国水稻起源进化研究

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选择原产中国的98份亚洲栽培稻和125份普通野生稻为材料,对叶绿体中atpA序列、rps16内含子序列、trnP-rp133间隔区、trnG-trnfM序列、trnT-trnL间隔区序列的5段高突变序列进行测序,利用生物信息学方法进行比对分析,绘制Network网络图,构建系统发育树.结果表明,普通野生稻的Indel和SNP数目均比亚洲栽培稻多,序列多样性丰富;基于单倍型的Network网络图和系统发育树可将所有参试材料归为3个类群,类群Ⅰ主要为粳稻与普通野生稻,类群Ⅱ主要为籼稻,类群Ⅲ主要为普通野生稻,而类群Ⅱ和类群Ⅲ亲缘关系较近,提示粳、籼两个亚种可能由偏粳、偏籼的普通野生稻分别进化而来,支持二次起源学说;所有与亚洲栽培稻亲缘关系较近的普通野生稻均来源于华南地区,支持华南地区为我国亚洲栽培稻起源中心的论点.
Origin and Evolution of Cultivated Rice (O.sativa L.) in China Based on Gene Diversity of Chloroplast Genome
To study the origin and evolution of Asian cultivated rice ( Oryza sativa L. ) ,98 accessions of Asian cultivated rice and 125 accessions of common wild rice {Oryza rufipogon) were selected for sequencing analysis. Through sequencing the atpA gene,rpsl6 intron,trnP-rpl33 inter spacer,trnG-trnfM inter spacer and trnT-trnL inter spacer in chloroplast genome in rice, the haplotype network and phylogenetic tree were made with bio-information software. The results showed that common wild rice had more Indels and SNPs compared with the Asian cultivated rice. Both the haplotype network and phylogenetic tree divided all test materials into three groups. Group I consisted by accessions from the Japonica subspecies and the common wild rice, Group II included mainly accessions of Indi-ca subspecies, while Group III were as the accessions of the common wild rice. Importantly, Group II and Group HI had a close relationship, which indicated that the Japonica and Indica Subspecies were originated from Japonica-like and Indica-like common wild rice separately, supporting the Double Domestication Hypotheses of Asia cultivated rice. All the materials of common wild rice with a close relationship to Asian cultivated rice were identified to be collected from South China, implicating South China might be the origin center of Asian cultivated rice.

RiceChloroplastGene diversityOriginIndica-japonica differentiation

王荣升、魏鑫、曹立荣、乔卫华、张万霞、杨庆文

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中国农业科学院作物科学研究所,北京100081

水稻 叶绿体 基因多样性 起源 籼粳分化

农业部财政专项转基因重大专项

2008ZX08011-001

2011

植物遗传资源学报
中国农业科学院作物科学研究所 中国农学会

植物遗传资源学报

CSTPCDCSCD北大核心
影响因子:1.415
ISSN:1672-1810
年,卷(期):2011.(5)
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