Introducing Phytase Gene phyA into Different Soybean Varieties from Transgenic Line Utilizing Sexual Hybridization
Phosphorus ( P) is one of the most important inorganic nutrients that can significantly affect plant growth and metabolism. However,30 to 40 percent of the unavailable P in agricultural soils exists as phytate,which can not directly be absorbed by plant except for resolved by phytase. So selecting or developing new varieties that can resolve phytate-P through the modern transgenic breeding approach provided a new opportunity to improve the efficiency of phosphorus by plants. In this paper,a phytase gene(phyA) , isolated from Aspergillus ficuum, was introduced into soybean by Agrobacterium-mediated transformation, and then thirty-eight soybean crosses had been made between transgenic lines(JL35-phyA,male parent)and other non-transgenic varieties(female parent). PCR and RT-PCR results showed that the phyA was successfullly incorporated into soybean genome and expressed in transgenic line JL35-phyA. Futhermore,the pods,seeds,and weights per plant and weight 100 seeds of JL35-phyA were higher than wildtype JL35 significantly. The results also showed that 427 PCR positive transgenic F1 plants from the thirty-eight cross combinations above and 377 PCR positive transgenic F2 plants from the progenies of the cross combination JD17 × JL35-phyA were obtained by the sexual hybridization transfer method. Analysis results of these progenies showed that the variation of agronomic characters were abundant,and some excellent transgenic lines were selected. So it provides many important genetic resources to transgenic breeding of phyA in soybean.
NETL
NSTL
万方数据
维普
