Isolation of Phloem Protein Gene and Expression Analysis in Celery
The phloem proteins play important role in the maintenance of morphology, material transportation and wound protection in higher plant.Here,cDNA sequences of phloem protein were obtained using RT-PCR technology from two celery varieties Liu He Huang Xin Qin and Mei Guo Xi Qin.The full-length of the two phloem-protein genes were 546 bp, and encoded 181 amino acid residues, respectively.The nucleotide sequences alignment showed that there were 3 loci differences: 88G/A,399T/C and 489T/C.The deduced amino acid sequences showed that there was 1 residue difference,30T/A.Prediction of the proteins molecular weight were 19 kDa, and pI were 9.18, respectively.The P-proteins from Liu He Huang Xin Qin and Mei Guo Xi Qin have highly identity with Lycoris aurea.There were 5 leucine and 4 tryptophan amino acid residues in the conservative position, respectively.Real time RT-PCR analysis showed that the genes expression were tissue specificity,which mainly expressed in the stem and root.
NETL
NSTL
万方数据
维普
