Genetic Diversity of Pepper Germplasm Investigated by EST-SSR Markers
Pepper (Capsicum annuum L.) is one of the staple vegetable crops with significant economic value.Investigation of pepper genetic diversity is crucial for the collection,identification,and application of pepper germplasm resources.EST-SSR markers,derived from the transcript regions,can detect the polymorphism in the transcript regions on the genome and thus exhibit the true genetic diversity.In the study,a total of 33 EST-SSR markers were employed to investigate the genetic diversity of 31 pepper varieties.A total of 91 alleles were detected among the tested pepper varieties by the EST-SSR markers.The maximum number of alleles and the average alleles per locus were 6 and 2.76,respectively.The polymorphic information content (PIC) per locus ranged from 0.03 to 0.74 with the average of 0.38.Systemic cluster analysis separated the tested pepper varieties into two major groups which showed certain correlation with the morphologic traits of the fruit.The result of principal coordinate analysis was similar to that of the systemic cluster analysis,exhibiting more thoroughly genetic variations among the tested pepper varieties.
NETL
NSTL
万方数据
维普
