Genetic Diversity Analysis of the Sea Island Cotton Introduced Using SSR Markers
The genetic relationship and genetic diversity of 56 Sea-island cotton (Gossypium barbadense L.) were analyzed by 95 pairs of SSR markers.Among the 56 sea-island cotton varieties,33 came from former USSR,2 from USA,1 from Albania,8 from Xinjiang,and 7 from Yunnan,Jiangsu,and other places of China.The 95 pairs of polymorphic SSR primers produced 384 alleles in total,and 296 were polymorphic,which accounted for 77.1％.2 to 9 alleles could be obtained from each pair of primers with an average of 4.1.The gene diversity(H)was 0.035 to 2.931 with an average of 0.879.Shannon's information index(Ⅰ)was 0.09 to 4.417 with an average of 1.363.The results showed the same trend.The value of allelic polymorphism information content (PIC) ranged from 0.035 to 0.926,with an average of 0.698.The result of UPGMA cluster showed that four groups could be clearly clustered when genetic similarity coefficient was 0.69.The first group contained 27 varieties,mainly from former USSR.But Giza 77,Paizi cotton,and Zhong 239 were assigned to this group.The second group was a little complicated and mostly included 6 varieties from Xingjiang and 4 varieties from Egypt.The third group contained 8 varieties,6 were from former USSR,Ba3116 from Xinjiang,and JI B91-45 from Hebei.The last group contained only one variety L-8007 from former USSR.Our results showed that SSR as a kind of codominant markers,could be used for the pedigree analysis and identification of sea-island especially.Therefore,SSR markers could provide a reliable basis in the molecular level for sea-island cotton breeding practice of matching parents.
NETL
NSTL
万方数据
维普
