In order to study the relationship between elevation and populations of Populus szechuanica var.tibetica located on the Qinghai-Tibet Plateau,24 pairs of SSR primers were used to study genetic diversity and structure of populations in Sejila Mountain of the southeastern plateau.A total of 126 alleles was detected from 469 individuals,with an average of 5.25 alleles per locus,a PPL(polymorphism percentage level) of 100%,and a relatively high expected heterozygosity (He) for populations at high and low altitudes of 0.48 and 0.49,respectively.Analysis of molecular variance(AMOVA) showed that differentiation among populations accounted for 6.38% of the total and most variation existed among the individuals within a population.A differentiation coefficient (FST) of 0.02 and the value of gene flow (Nm) was 9.89 at a very high level also supported the conclusion that the differentiation among populations was at a low level.In conclusion,no geographical separation and differentiation of populations along the altitude of Sejila Mountain were observed.As a result,conservation and utilization of Tibetan poplar facilitated on the basis of understanding of differentiation and distribution patterns of the species and study on mechanism of adaptation to a high altitude could be proceed smoothly.
Populus szechuanica var.tibeticagenetic diversitypopulation structurealtitudeQinghai-Tibet Plateau