Proteomic Analysis of Storage Substances during after-ripening of Dormant Seeds with Dry Ripening Process in Maize Inbred Line 08-641
Maize inbred line 08-641 was used as material to analyze the proteome difference during seed dormancy breaking by mean of 2-D electrophoresis method.The results showed that 600 protein spots were detected in fresh dormant seed and 620 protein spots were detected when seed dormancy was broken through 10 d after-ripening,respectively.Among the 23 differential protein expressions spots,there were 4 down-regulated protein spots,4 up-regulated proteins,8 protein spots with additional expression,and 7 protein spots with missing expression.The proteins identified by MALDI-TOF-MS were main included globulin,late embryogenesis abundant protein,legumin and other storage proteins,proteasome and sorbitol dehydrogenase involved in metabolism,heat shock protein involved in protein structure and cell function regulation.The seed dormancy of maize inbred line 08-641 was due to lack of expression or over-expression of some dormancy-related proteins.
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NSTL
万方数据
维普
