Southern rust is an important disease in maize production.A total of 903 maize accessions were screened for resistance to Southern Corn Rust in Nanning,Guangxi and Changping,Beijin from 2013 to 2015.And the SSR markers were adopted in genetic diversity analysis of the selected partially resistant materials.In Nanning,Guangxi and Changping,Beijing,both of the results showed that among the 903 germplasm,8 inbred lines were highly resistant to southern corn rust,accounting for 0.88％ of the total germplasm.Twenty-nine germplasm,including 27 inbred lines and 2 landraces were resistant to this disease,accounted for 3.2％ of total accessions,100 germplasm was moderately resistant,accounted for 3.2％ of total accessions,181 and 585 accessions was susceptible and highly susceptible to southern corn rust,accounting for 20.0％ and 64.8％ of the total materials,respectively.It can be seen that maize germplasm with high resistance to southern corn rust is relatively scarce and hence the highly resistant accessions expressed in both areas are the valuable resources.There exists difference in resistance to southern corn rust among maize germplasm from different geographical areas,the resistant germplasm was most frequently screened from Inner Mongolia,followed by Shanxi.Fourty-two pairs polymorphic of SSR primers were detected and a total of 141 alleles were amplified in the 50 accessions,including 139 polymorphic bands,with polymorphic site percentage (PPB) of 98.58％.The average number of alleles (Na) was 1.98,the average effective number of alleles(Ne) was 1.59,with the average Nei's gene diversity (H)of 0.34.The average polymorphism information content (PIC) and Shannon's information index (Ⅰ) were 0.78 and 0.51,respectively.Using UPGMA analysis,the 50 maize accessions were classified into two groups of which the first group contains five heterosis subgroups with relatively high genetic diversity,which can provide reference information for the selection and utilization of resistance sources in disease resistance breeding.
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维普
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