高粱株高相关基因SbPH11分子标记的开发和应用
Development and Application of Molecular Markers of Sorghum Plant Height Related Gene SbPH11
段国旗 1吕娜 1石颖怡 2张怀 3李斌峰 3侯留飞 4许文秀 2闫慧莉 2何振艳 2平俊爱5
作者信息
- 1. 山西农业大学高粱研究所/省部共建有机旱作农业国家重点实验室(筹),晋中 030600;山西农业大学农学院,太谷 030801
- 2. 中国科学院植物研究所,北京 100093
- 3. 青海牛必乐农牧科技有限公司,西宁 810003
- 4. 青海省饲草料技术推广站,西宁 810003
- 5. 山西农业大学高粱研究所/省部共建有机旱作农业国家重点实验室(筹),晋中 030600
- 折叠
摘要
生物量是饲用高粱的重要性状,株高与生物量呈正相关性.本研究以237份高粱自交系关联群体为材料,筛选到株高关联基因SbPH11的两个功能性SNP位点,两个SNP位点组合的单倍型共3种:SbPH11-Hap1、SbPH11-Hap2和SbPH 11-Hap3,SbPH11-Hap2所对应的高粱材料的株高极显著高于SbPH 11-Hap 1和SbPH11-Hap3所对应的高粱材料的株高,SbPH11-Hap1的高粱材料株高极显著高于SbPH11-Hap3的高粱材料株高.针对SbPH11的两个功能性SNP位点开发了 KASP分子标记,利用该标记对30份高粱种质资源进行了基因分型和表型验证,结果证实开发的KASP分子标记可以准确地鉴定出SbPH11两个功能性SNP位点的基因型.该KASP分子标记可高效准确地预测不同高粱种质资源的株高类型,可应用于高粱株高的早期筛选和分子标记辅助选择育种.
Abstract
Biomass is an important trait in forage sorghum,and positively correlates with plant height.In this study,two functional SNPs of the plant height associated gene SbPH11 were screened from 237 sorghum inbred lines.Three haplotypes,named SbPH11-Hap1,SbPH11-Hap2 and SbPH11-Hap3,at the SbPH11 locus with different combination of the two SNPs have been identified.The plant height of SbPH11-Hap2 materials was extremely significantly higher than that of SbPH11-Hap1 and SbPH11-Hap3 materials,and that of SbPH11-Hap1 materials was extremely significantly higher than that of SbPH11-Hap3 materials.The KASP marker that associates to plant height was developed based on two functional SNPs of the gene SbPH11.The marker was used to genotype and phenotype of 30 sorghum germplasms resources.The KASP marker was confirmed to accurately identify two functional SNPs of SbPH11.These results suggested that the newly-developed KASP marker can efficiently and accurately predict the plant height in sorghum germplasm resources,and can be applied in early screening and molecular marker-assisted selection in breeding for sorghum with higher plant height.
关键词
高粱/株高/KASP分子标记/SbPH11Key words
sorghum/plant height/KASP molecular marker/SbPH11引用本文复制引用
基金项目
西宁市重点研发与转化计划(2022-Y-18)
省部共建有机旱作农业国家重点实验室自主研发项目(202002-4)
中国科学院战略性先导科技专项(XDA26030201)
现代农业产业技术体系建设项目(CARS-06)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据