采用小米培养基接种法对163份当前推广的小麦种质资源进行室内和田间茎基腐病抗性鉴定.结果表明,没有鉴定出高抗和免疫的小麦材料,但是不同材料的抗性能被明显区分,群体抗性整体上呈现正态分布趋势,室内鉴定病情指数分布在13.28～83.33之间,田间鉴定病情指数分布在10.27～73.89之间.室内和田间鉴定结果较稳定,两环境病情指数的相关系数为0.79,说明室内鉴定结果可较好反映田间抗性情况.全基因组关联分析(GWAS,genome-wide association study)显示,显著SNP广泛分布于小麦各条染色体上,其中2A上最多,集中在725～763 Mb区段内.进一步集群分离分析法(BSA,bulked segregant analysis)结果显示,显著SNP集中在2A上的730～750Mb区段内.综合来看,小麦2A染色体上730～750Mb区段内可能存在显著调控小麦茎基腐病的抗性基因.本研究能够为小麦茎基腐病抗性材料筛选及抗病位点挖掘提供重要的参考意义.
The Resistance Investigation and Mapping Analysis of Fusarium Crown Rot for Wheat Accessions
By applying the millet grains as pathogen medium,we inoculated the 163 wheat germplasm accessions which widely cultivated currently.No immune or highly resistant wheat accessions were identified,while the resistance variations estimated by disease index(DI)were found following a normal distribution 13.28-83.33 in greenhouse and 10.27-73.89 in field,respectively.The correlation coefficient,based on the phenotype collected from greenhouse seedling assay and field trials was 0.79,supporting a high correlation on resistance at the seedling and adult stages.Furthermore,the genome-wide association study(GWAS)found significant SNPs distributed on all chromosomes of wheat,with enrichment on chromosome 2A with an interval from 725 Mb to 763 Mb.This was in keeping with the results of the bulked segregant analysis(BSA),which revealed significant SNPs distributed from 730 Mb to 750 Mb on chromosome 2A.GWAS and BSA indicated a significant resistance locus,which resided from 730 Mb to 750 Mb on chromosome 2A and associated with fusarium crown rot(FCR)infection.This study can provide important reference for identifying resistant wheat materials and exploring FCR resistant loci in wheat.
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