首页|生姜IPT基因家族的鉴定与表达分析

生姜IPT基因家族的鉴定与表达分析

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生姜是一种重要的"药食同源"植物,但其种植过程中容易受到各种生物/非生物胁迫的影响,不利于生姜的安全生产.异戊烯基转移酶(IPT,isopentenyl-transferases)是催化细胞分裂素生物合成的关键酶,也是重要的限速酶,与植物的抗逆性关系密切.本研究通过系统的生物信息学分析,从生姜基因组中鉴定到10个ZoIPT,并且将其命名为ZoIPT1～ZoIPT10.其编码蛋白的氨基酸长度范围在283～491 aa之间,分子质量在31.14～54.02 kDa,等电点在4.97～9.37之间;蛋白质特征分析表明,所有ZoIPTs均具亲水性.共线性关系分析发现生姜IPT基因与15个芭蕉IPT基因存在共线性,与1个拟南芥IPT基因存在共线性.转录组数据分析结果显示,ZoIPTs有一定的组织表达特异性,并且能响应病害、低温等逆境胁迫,其中,ZoIPT3和ZoIPT5在生姜不同生长时期、不同部位、低温和病害胁迫下均有较高表达.qRT-PCR分析结果表明,ZoIPTs响应干旱、淹水、盐胁迫.在淹水和盐胁迫下,根茎中ZoIPT3表达量显著上升;在干旱胁迫下,叶和根茎中ZoIPT5的表达显著上升.综上所述,本研究通过系统的鉴定、进化分析、特征分析、启动子分析、表达模式分析,并对干旱、盐、淹水胁迫下的表达模式进行了分析,为深入研究ZoIPT在调控生姜生长发育和抗逆性中的生物学功能提供了理论基础.
Identification and Transcriptional Profile Analysis of IPT Gene Family Members in Ginger
Ginger is an important vegetable crop that can be used as medicine and food,but it is sensitive to various biotic and abiotic stresses during its cultivation,which is detrimental to the safe production.Isopentenyl-transferases(IPTs)are key enzymes that catalyze the biosynthesis of cytokinin and are also important rate-limiting enzymes of cytokinin,and it is closely related to the stress resistance of plants.Here,ten ZoIPTs had been identified from ginger genome through the bioinformatics analysis,designated ZoIPT1～ZoIPT10.The encoded protein ranged from 283 to 491 amino acids,with the molecular weight of 31.14 to 54.02 kDa,and the isoelectric point pI of 4.97 to 9.37.All these ZoIPTs were predicted to be hydrophilic.Transcriptome data analysis showed that ZoIPTs showed tissue-specific transcriptional patterns and could respond to stress treatments such as disease and low temperature.ZoIPT3 and ZoIPT5 were highly expressed at different growth stages,different tissues,and in response to low temperature and disease stress.qRT-PCR analysis showed that ZoIPTs was responsive to drought,flooding and salt stress.Under flooding and salt treatment conditions,the significant induction of ZoIPT3 in rhizomes was observed.Under drought stress,the expression of ZoIPT5 in leaves and rhizomes were significantly increased.In summary,with the results of the systematic identification,evolutionary analysis,characteristic analysis,promoter analysis,as well as transcriptional pattern analysis,and analyzed the expression patterns of ZoIPT genes under drought,flooding and salt stress,this study provided a theoretical basis for further in-depth research on the biological functions of ZoIPTs in regulating growth and development and stress responses in ginger.

Zingiber officinale Roscoe.IPTbioinformatics analysisexpression analysis

席克勇、田野、王艳红、刘奕清、尹军良、梁红艳、朱学栋、朱永兴

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长江大学园艺园林学院/长江大学香辛作物研究院,湖北荆州 434025

长江大学农学院,湖北荆州 434025

荆州农业科学院,湖北荆州 434025

生姜 IPT 生物信息学分析 表达分析

湖北省重点研发计划项目湖北省重点研发计划项目荆州市2022年度科技计划重庆英才·优秀科学家项目Key Research and Development Program of Hubei Province,ChinaKey Research and Development Program of Hubei Province,China

2021BBA0972022BBA00612022BB36CQYC202201015142022BBA00612021BBA096

2024

10.13430/j.cnki.jpgr.20230802002

植物遗传资源学报
中国农业科学院作物科学研究所　中国农学会

植物遗传资源学报

CSTPCD北大核心
影响因子：1.415
ISSN：1672-1810
年,卷(期)：2024.25(2)
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