Comprehensive Evaluation on Saline-alkali Tolerance of 286 Brassica napus Germplasm at Seedling Stage
The salt-alkali tolerance of 286 brassica rapeseed strains at the seedling stage was identified in this study.The indexes of leaves number,green leaves number,green leaves rate,shoot length,root length,and root weight under salt-alkali stress were determined through a hydroponics test.Various methods including comprehensive assessment value(D-value),extreme material screening analysis,correlation analysis,principal component analysis,membership function analysis,frequency analysis and stepwise regression analysis were used.A comprehensive evaluation model for saline-alkali tolerance was established for different genotypes of rapeseed germplasm at the seedling stage and appropriate evaluation indexes were selected.Under saline-alkali stress conditions,leaves number showed a negative correlation with plant height but the correlation coefficient between them was not significant;however other traits showed positive correlations that reached a significant or extremely significant level.Principal component analysis was employed to synthesize 7 salt-alkali tolerance indexes into 4 principal components which could represent 88.349％of the original data information on rape's salt-alkali tolerance.Based on the relative importance(weight)of these four principal components,a comprehensive evaluation value(D-value)for salt and alkali tolerance among different genotypes was obtained.Four alkali-salt-tolerant brassica napus germplasms and four saline-sensitive germplasms were screened using membership function analysis and extreme material screening analysis respectively.The results from stepwise regression analysis indicated that salinity tolerance in brassica napus germplasm resources could be determined by measuring the green leaves number,green leaves rate,shoot weight as well as root length and root weight at the seedling stage while estimating D-value using regression equations.
Brassica napusseedling stagesaline-alkali stressscreening of germplasm resourcescomprehensive evaluation
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