Cloning and Functional Study of Cryptochrome Gene CoCRY1 from Camellia oleifera Abe.
Camellia oleifera Abe.is an important woody oil tree in China,which has important economic and social benefits.Cryptochrome is one of the blue light receptors in plants,which is involved in the growth and development of plants such as flowering regulation and photomorphogenesis.In this study,CoCRY1 gene was cloned from C.oleifera'Huashuo'.Bioinformatics analysis showed that CDS sequence length of CoCRY1 was 2262 bp encoding for 684 amino acids,the molecular formula of the CoCRY1 protein was C3454H5281N971O1027S18,and the molecular weight was 77.42 kDa,further analysis showed that this protein sequence contained three domains,namely DNA_photolyase,FAD_binding_7 and Cryptochrome_C,which proved that CoCRYl protein belongs to cryptochrome family.Homologous sequence and phylogenetic tree analysis showed that CoCRYl protein had the highest similarity with the homologous sequence of tea tree(Camellia sinensis).Tissue expression analysis showed that the transcripts of CoCRY1 were the highest in stem and the lowest in flower.Arabidopsis thaliana plants with heterogenous expression of CoCRY1 gene were obtained by Agrobacterium transformation,and transgenic plants were identified from DNA and RNA level.Phenotypic analysis of transgenic Arabidopsis showed that overexpression of CoCRY1 gene induced early flowering under long day londition and had photospectific inhibitory effect on hypocotyl elongation.Through bioinformatics,quantitative analysis and heterologous expression,it was found that CoCRY1 gene plays an important role in the biological process of C.oleifera flowering.
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