首页|基于选择清除分析挖掘连翘异型花柱候选基因

基于选择清除分析挖掘连翘异型花柱候选基因

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连翘(Forsythia suspensa(Thunb.)Vahl)为多年生木本植物,花柱二型具有自交不亲和性.挖掘连翘异型花柱发育候选基因,利于揭示连翘异型花柱的进化与发育机理.对40株长花柱型与短花柱型连翘植株进行全基因组重测序,检测样本基因组的SNP变异位点,开展选择清除分析.利用遗传分化系数Fst和核苷酸多态性π ratio结合的方法筛选候选区域(前5％水平),对差异基因进行GO数据库和KEGG数据库功能注释分析,采用qRT-PCR对候选基因在花柱发育不同时期的表达量进行检测.选择清除分析显示受选择基因295个,主要在植物的生长发育以及代谢调控方面发挥重要作用.分析植物激素调节相关的部分通路,确定连翘异型花柱候选基因CYP734A1(EVM0010386)、BRH(EVM00H829)、CYCD3(EVM0018316),基因表达结果显示CYP734A1基因在连翘短花柱发育的关键时期持续高表达,露冠期BRI1与CYCD3基因表达水平在短花柱型中显著高于长花柱型.本研究挖掘的候选基因在连翘长花柱与短花柱中存在差异表达,油菜素甾醇参与了连翘异型花柱的发育,是产生连翘花柱异型的原因之一.本研究为连翘异型花柱的进化以及遗传发育调控机制解析奠定基础并提供思路.
Based on Selective Sweep Analysis Mining Candidate Genes of Heterostyly in Forsythia suspensa
Forsythia suspensa(Thunb.)Vahl is a perennial woody plant with dimorphic style and self-incompatibility.Mining the candidate genes for the development of heterostyly in Forsythia suspensa is of significance to reveal its evolution and development mechanism.The whole genome resequencing of 40 long style morph and short style morph Forsythia suspensa plants were conducted,followed by the SNP variation sites detection in the sample genome and the selection sweep analysis.The candidate regions(top 5％level)were screened by the combination of genetic differentiation coefficient Fst and the nucleotide polymorphism π ratio.Differentially expressed genes(DEGs)were analyzed by GO and KEGG enrichment annotation,and qRT-PCR was used to detect the transcriptional profiles of candidate genes at different periods of style development.Selective sweep analysis showed that 295 genes were under selection,which mainly played an important role in plant growth and development and metabolic regulation.By analyzing the pathways in plant hormone regulation,the candidate genes CYP734A1(EVM0010386),BRI1(EVM0011829)and CYCD3(EVM0018316)of heterostyly in Forsythia suspense were analyzed.The gene expression results indicated that the CYP734A1 gene has sustained high expression during the critical period of development in F.suspensa's S-morph flowers.The BRI1 and CYCD3 genes in the S-morph were significantly higher than those in the L-morph during the crown exposure period.The candidate genes excavated in this study were differentially expressed in the L-morph and S-morph style of F.suspensa.Brassinosteroids was involved in the development of the heterostyly in F.suspensa,which was one of the reasons for heterostyly of F.suspensa.Collectively,this study laid a foundation for the evolution and genetic development regulation mechanism of heterostyly in F.suspensa.

Forsythia suspensa(Thunb.)Vahlheterostylywhole genome resequencingselection sweep analysisexpression analysis

杜晓蓉、李琦、李澳旋、樊阿梅、何龙娇、靳亚鑫、张灵灵、乔永刚、宋芸

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山西农业大学生命科学学院,太谷 030801

山西农业大学中兽医药现代化山西省重点实验室,太谷 030801

连翘 异型花柱 全基因组重测序 选择清除分析 表达分析

山西省基础研究计划自然科学研究面上项目山西省现代农业中药材产业技术体系项目(2023)山西农业大学科技创新提升工程项目山西省高等学校大学生创新创业训练计划项目

20210302123390CXGC202304120220172

2024

10.13430/j.cnki.jpgr.20230907001

植物遗传资源学报
中国农业科学院作物科学研究所　中国农学会

植物遗传资源学报

CSTPCD北大核心
影响因子：1.415
ISSN：1672-1810
年,卷(期)：2024.25(3)
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