托桂型菊花花型和花色性状遗传规律分析
Analysis of Genetic Law of Inflorescence Traits and Floret Color of Anemone-type Chrysanthemum
张一驰 1叶雨晴 1辜源馨 1张德平 2牛雅静 3张蒙蒙 3黄河1
作者信息
- 1. 北京林业大学园林学院/花卉种质资源创新与分子育种北京市重点实验室/国家花卉工程技术研究中心/城乡生态环境北京实验室,北京 100083
- 2. 成都市龙泉驿区农业农村局,成都 610100
- 3. 北京市植物园管理处/北京市花卉园艺工程技术研究中心,北京 100093
- 折叠
摘要
以一个白色非托桂型小菊为父本,两个红色托桂型小菊为母本,设计两个杂交组合,测定了杂交F1的11个花型性状.遗传分析表明,花径、舌状花长、舌状花宽和管状花数均符合无主基因控制的0MG模型;心花直径、舌状花数、管状花长、管状花宽和最深齿裂长均符合由两个加性-显性-上位性效应的主基因控制的2MG-ADI模型,其中管状花长和最深齿裂长的主基因遗传率均约70％,属于高度遗传力.相关性分析表明,托桂型菊花管状花长、管状花宽和最深齿裂长两两之间呈极显著的正相关.根据舌状花的花色值(L*,a*,b*)将F1聚类为5个不同色系,两个杂交组合舌状花花色的香农-威纳指数(H)分别为1.12和1.23,花色变化丰富.此外,测定了亲本及F1共30个不同花色的托桂型菊花管状花内侧表皮和舌状花上表皮的花色值(L*,a*,b*),发现两者的L*,a*,b*值分别呈极显著正相关;对5个色系共10个托桂型菊花做切片观察,发现管状花内侧与舌状花上表皮、管状花外侧与舌状花下表皮的色素种类相同且细胞形态大小相似,因而花色相近.本研究结果为菊花托桂花型性状的基因定位以及优质托桂型菊花品种的培育提供理论依据.
Abstract
In this study,two F1 hybrid combinations via crossing white non-anemone-type chrysanthemum(male)with two red anemone-type chrysanthemums(female)were used to determine 11 inflorescence traits.Genetic analysis revealed that flower diameter,ray floret length,ray floret width and number of disc florets were accordant with the 0MG model without major gene control.Disc flower diameter,number of ray florets,disc floret length,disc floret width and the longest lobe length fitted to the 2MG-ADI model controlled by two major genes with add-demonstration-epistatic effect.Major genes for disc floret length and the longest lobe length had a heritability of approximately 70％,indicating high heritability.Correlation analysis revealed significant positive correlation among disc floret length,disc floret width and the longest lobe length.According to the color values(L*,a*,b*)of ray florets,F1 populations were grouped into 5 different colors.The Shannon-Wiener index(H)of the ray florets of two hybrid combinations were 1.12 and 1.23,the colors varied widely.Additionally,we selected 30 anemone-type chrysanthemums with different colors from the parents and F,population and measured the flower color values(L*,a*,b*)of inside epidermis of disc florets and upper epidermis of ray florets.The results suggest a respectively significant positive correlation of L*,a*and b*values between the two.Through examining 10 anemone-type chrysanthemums with 5 different colors,the same pigment as well as similarity on cell morphology and size were observed between inside epidermis of disc florets and upper epidermis of ray florets,as well as outside epidermis of disc florets and lower epidermis of ray florets possess.The results provided a theoretical foundation for the genetic mapping of inflorescence traits and the cultivation of high-quality anemone-type chrysanthemum varieties.
关键词
菊花/托桂花型/花型性状/遗传规律/花色分析Key words
chrysanthemum/anemone type/inflorescence traits/genetic law/floret color analysis引用本文复制引用
基金项目
北京市自然科学基金(6212022)
大学生创新创业训练计划(202210022030)
出版年
2024
国家科技期刊平台
NSTL
万方数据