摘要
通过目测法及比色卡进行表型观测并选取8种不同花色的蔷薇属植物作为研究对象,利用分光色差仪测定花色表型,采用高效液相色谱质谱联用技术对花青素苷进行定性定量分析,探讨蔷薇属植物花色与花青素苷之间的关系.结果表明,8种蔷薇属植物材料首次检测出8种花青素苷成分,分别为矢车菊素-3-(咖啡酰基)-葡萄糖苷(Cy3CafG)、矢车菊素-3-O-半乳糖苷(Cy3Gal)、芍药素-3-(咖啡酰基)-葡萄糖苷(Pn3CafG)、矢车菊素-3-(顺式-咖啡酰基)-二甲基葡萄糖苷(Cy3(cisCaf)DmG)、矢车菊素-3-(反式-咖啡酰基)-二甲基葡萄糖苷(Cy3(transCaf)DmG)、矢车菊素-3-二甲基-葡萄糖苷(Cy3DmG)、芍药素-3-(顺式-咖啡酰基)-芸香苷(Pn3(cisCaf)Ru)、芍药素-3-(反式-咖啡酰基)-芸香苷(Pn3(transCaf)Ru).8种花青素苷的生物修饰类型包含半乳糖糖基化、甲基化修饰和咖啡酰基化修饰3种,这3种生物修饰类型均在蔷薇属植物中首次报道.花青素苷与CIELab参数相关性结果显示Cy3CafG、Cy3(cisCaf)DmG与花瓣红度(a*a)呈正相关,Cy3Gal、Cy3(transCaf)DmG与花瓣黄度(b*)和亮度(L*)呈显著正相关,相关性系数均大于0.5.本研究为蔷薇属植物中花青素苷的精准鉴定和花色呈色机理研究提供理论参考,为蔷薇属植物分子育种提供方法基础.
Abstract
In this study,eight Rosa plants showing different flower colors were subjected for phenotypic observation using the CIELab color measurement system.The anthocyanins were qualified using the high-performance liquid chromatography-mass spectrometry(HPLC-MS/MS)techniques,and examined for the relationship with the flower colors.The results showed that eight anthocyanins components were first detected,including Cyanidin-3-(caffeoyl)-glucoside(Cy3CafG),Cyanidin-3-O-galactopyranoside(Cy3Gal),Peonidin-3-(caffeoyl)-glucoside(Pn3CafG),Cyanidin-3-(cis-caffeoyl)-dimethyl-glucoside(Cy3(cisCaf)DmG),Cyanidin-3-(trans-caffeoyl)-dimethyl-glucoside(Cy3(transCaf)DmG),Cyanidin-3-dimethyl-glucoside(Cy3DmG),Peonidin-3-(cis-caffeoyl)-rutinoside(Pn3(cisCaf)Ru),Peonidin-3-(trans-caffeoyl)-rutinoside(Pn3(transCaf)Ru).The anthocyanin glycosides include galactosylation,methylation modification,and caffeioylation modification,all of which have been reported for the first time in Rosa plants.The correlation analysis between anthocyanins and CIELab parameters showed that Cy3CafG and Cy3(cisCaf)DmG were positively correlated with petal redness(a*).Cy3Gal and Cy3(transCaf)DmG were significantly positively correlated with petal yellowness(b*)and luminance(L*).This study provides a theoretical reference for the precise identification of anthocyanins in Rosa plants and the study of the mechanism of flower color formation,and provides a methodological basis for molecular breeding of Rosa plants.
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