大豆籽粒中氨基酸含量丰富，是大豆品质的重要组成部分，具有较高的营养价值和生理功能。本研究利用高效液相色谱法测定264份大豆品种干籽粒中精氨酸、甘氨酸、谷氨酸、赖氨酸等4种游离氨基酸含量。结果表明，游离氨基酸中精氨酸含量最高、谷氨酸含量次之、甘氨酸含量最低，筛选出四种游离氨基酸均高含量的3个大豆优质品种，分别为海门羊104、辽鲜豆12号、灌云大四粒。结合大豆自然群体四种游离氨基酸含量和基因型进行全基因组关联分析，大豆四种氨基酸2年均可定位到的显著SNP位点共27个，其中精氨酸有2个SNP位点，包括S17_19067780和S17_19067789，甘氨酸有19个SNP位点，包括 S01_53974257、S08_38878988 等，谷氨酸有 1 个 SNP 位点，S18_53291599，赖氨酸有 5 个 SNP 位点，包括 S08_18555689、S08_18567542等;并推测大豆氨基酸高含量相关候选基因，精氨酸为Glyma。17G177400和Glyma。17G177600，甘氨酸为Glyma。11G157000 和 Glyma。11G161700，谷氨酸为 Glyma。18G244200 和 Glyma。18G244700，赖氨酸为 Glyma。08G227600 和Glyma。08G228100。本研究结果为大豆品种改良、辅助大豆分子育种提供理论基础。
Identification and GWAS Analysis of Soybean Germplasm with High Content of Four Free Amino Acids
The amino acids are rich in soybean grains and are important components of the grain quality.In this study,264 soybean genotypes were analyzed using high-performance liquid chromatography(HPLC)to determine the levels of four free amino acids:arginine,glycine,glutamic acid,and lysine in dried soybean grains.Among the free amino acids,arginine was detected with the highest content,followed by glutamic acid,and glycine.Three soybean varieties with high levels of these four free amino acids were identified,including Haimen Yang 104,Liaoxian Bean 12,and Guanyun Dasiyu.By genome-wide association analysis combined with the phenotypes and genotypes of four free amino acids in soybean natural population,27 significant SNPs co-located by four amino acids in soybean were identified in two years,among which there were two SNPs for arginine,including S17_19067780 and S17_19067789,and 19 SNPs for glycine,including S01_53974257,S08_38878988,et al,one SNP(S18_53291599)for glutamic acid,five SNPs for lysine,including S08_1 8555689,S08_18567542,et al.The candidate genes related to high amino acid content in soybean were inferred to be Glyma.17G177400 and Glyma.17G177600 for arginine,and Glyma.11G157000 and Glyma.11G161700 for glycine.Glutamic acid are Glyma.18G244200 and Glyma.18G244700,lysine are Glyma.08G227600 and Glyma.08G228100.The results of this study provide a theoretical basis for improving soybean varieties and assisting soybean molecular breeding.
soybeanfree amino acidgenome-wide association analysisSNP
NETL
NSTL
万方数据
