摘要
番茄作为重要的蔬菜作物之一,鉴定番茄种质资源的抗旱性及筛选抗旱番茄种质资源对番茄育种具有重要意义.本研究利用12.5％PEG-6000溶液对92份番茄种质资源进行干旱胁迫处理,测定发芽势、发芽率、发芽指数、活力指数、根长、芽长等6个抗旱性相关指标,运用相关性分析、主成分分析、隶属函数法和聚类分析进行番茄萌发期抗旱性评价及筛选.结果表明,干旱胁迫下6个指标均显著降低,其中活力指数和发芽势降低最大.通过主成分分析将6个指标转化为2个新指标,累计贡献率达86.657％.基于相关性和主成分分析,筛选出发芽势、发芽率、发芽指数、活力指数作为萌发期抗旱性鉴定的主要指标,利用隶属函数法结合聚类分析将番茄种质资源分为4类,其中抗旱型19份、较抗旱型41份、较敏感型18份、敏感型14份;通过增大、减小PEG-6000溶液浓度,进一步筛选出抗旱性强的密植红番茄和锦农矮秧两个品种,敏感性强的北京早红和大红袍两个品种.本研究结果可为番茄种质资源的抗旱性研究提供理论基础.
Abstract
Tomato,being one of the important vegetable crops,holds great significance in evaluating the drought resistance of tomato germplasm resources and selecting drought-resistant varieties for tomato breeding.In this study,we subjected 92 tomato germplasm resources to drought stress treatment using a 12.5％PEG-6000 solution.Six drought-related indexes,including germination potential,germination rate,germination index,vigor index,root length and shoot length,were measured,and the drought resistance of tomato at germination stage was evaluated and screened by correlation analysis,principal component analysis,membership function method and cluster analysis.The results showed a significant reduction in all six indicators under drought stress,with the vigor index and germination potential being the most affected.Through principal component analysis,we transformed the six indicators into two new indicators,which contributed to a cumulative contribution rate of 86.657％.Based on correlation and principal component analysis,we identified germination potential,germination rate,germination index,and vigor index as the main indicators for evaluating drought resistance during the germination period.By using the subordination function method combined with cluster analysis,we classified the tomato germplasm resources into four types,including 19 drought-resistant types,41 relatively drought-resistant types,18 relatively sensitive types,and 14 sensitive types.By adjusting the concentration of PEG-6000 solution,further screening of the drought-resistant varieties Mizhihong Tomato and Jinnongaiyang,as well as the sensitive varieties Beijingzaohong and Dahongpao can be conducted.These findings provide a theoretical basis for the study of drought resistance in tomato germplasm resources.
基金项目
新疆维吾尔自治区重大科技专项(2022A02005-2)
新疆蔬菜产业技术体系(XJARS-07)
四川省自然科学基金项目(2022 NSFSC1759)
国家科技期刊平台
NSTL
万方数据