摘要
本实验室前期以东乡普通野生稻和日本晴为亲本创制了强耐盐染色体片段置换系CSSL91,本研究将其与日本晴和强耐盐种质Pokkati比较,结果显示CSSL91耐盐性与Pokkali相当.以CSSL91与日本晴构建的F2∶3群体为试验材料,日本晴和CSSL91为对照,以耐盐等级和幼苗存活率为指标.结果表明2个指标均成正态分布,QTL连锁定位分析共检测到5个耐盐相关QTL,分别分布于第4、9、10号染色体上,LOD值介于2.95～3.97,表型贡献率为9.83％～18.48％;其中耐盐等级QTL-qST4的表型贡献率最高,其定位在第4号染色体DX-C4-1～DX-S4-16标记间.分离群体分组分析法(BSA,bulked segregation analysis)分析检测到第4号染色体0～5.0 Mb区间有一个超过阈值的QTL,该区间与QTL-qST4重合,QTL连锁分析方法和BSA方法均在第4号染色体的0～5.0 Mb区间定位到耐盐等级QTL,说明QTL-qST4是可靠的耐盐位点;耐盐等级QTL-qST4-1和幼苗存活率QTL-qSSR4均定位在第4号染色体DX-C4-12和DX-C4-13标记间,LOD值分别为3.36和3.92,表型贡献率分别为13.97％和9.49％;在第9号、10号染色体还定位到两个耐盐等级QTL-qST9和QTL-qST10;其中QTL-qST4-1、QTL-qSSR4和QTL-qST10是本研究新定位的耐盐性QTL.本研究结果将为水稻耐盐性相关基因克隆和分子标记辅助改良水稻品种的耐盐性奠定基础.
Abstract
In our laboratory,we created a chromosome fragment substitution line CSSL91,with high salinity-tolerance,using Dongxiang common wild rice and Nipponbare as parents.In this study,it was compared with Nipponbare and Pokkali,a strong salt-tolerant germplasm,and the results showed that the salt tolerance of CSSL91 was comparable to that of Pokkali.Using the F2∶3 population constructed by CSSL91 and Nipponbare,the phenotypes were normally distributed with salt tolerance grade and seedling survival rate as indicators.QTL linkage location analysis showed that a total of five salinity tolerance-related QTLs were detected,which were distributed on chromosomes 4,9,and 10,with the LOD values ranging from 2.95 to 3.97,and the phenotypic contribution rates ranging from 9.83％to 18.48％.Among that QTL-qST4 is the highest phenotypic contribution in the salt tolerance grade,which was located between DX-C4-1 and DX-S4-16 markers on chromosome 4.Simultaneously,the bulked segregation analysis(BSA)detected a QTL exceeding the threshold value in the interval of 0-5.0 Mb on chromosome 4,which was overlapped with QTL-qST4,indicating that QTL-qSST4 was a reliable salinity tolerance locus;QTL-qST4-1 and QTL-qSSR4 based on salt tolerance grade and seedling survival,respectively,were both located between markers DX-C4-12 and DX-C4-13 on chromosome 4,with LOD values of 3.36 and 3.92,and phenotypic contributions of 13.97％and 9.49％,respectively.Two QTLs based on salt tolerance grades,QTL-qST9 and QTL-qST10,were located on chromosomes 9 and 10,respectively.QTL-qST4-1,QTL-qSSR4 and QTL-qST10 are the new QTLs with salinity tolerance in this study.The results of the present study will lay a foundation for the cloning of salinity tolerance-related genes and molecular markers to assist in the improvement of rice varieties in terms of salt tolerance.
基金项目
科技创新2030-重大项目(2022ZD04017)
中国农业科学院科技创新工程项目()
国家科技期刊平台
NSTL
万方数据