大麦条纹病抗性鉴定及与SSR标记的关联分析
Barley Leaf Stripe Resistant Identification and Association Analysis with SSR Markers in Barley Germplasms
杨文娟 1司二静 1潘娇 1郭铭 1孟亚雄 1汪军成 1姚立蓉 1张宏 1李葆春 2马小乐 1王化俊1
作者信息
- 1. 干旱生境作物学国家重点实验室/甘肃省作物遗传改良与种质创新重点实验室,兰州 730070;甘肃农业大学农学院,兰州 730070
- 2. 干旱生境作物学国家重点实验室/甘肃省作物遗传改良与种质创新重点实验室,兰州 730070;甘肃农业大学生命科学技术学院,兰州 730070
- 折叠
摘要
大麦条纹病是对大麦产量及品质影响最为严重的病害之一,为探明我国不同来源的大麦种质对条纹病的抗性差异并挖掘与大麦抗条纹病相关联的候选标记,本研究利用97个SSR标记对137份大麦品种进行遗传多样性及群体结构分析,并结合抗性鉴定结果进行关联分析.结果表明,人工接种大麦条纹病菌后共鉴定出18份免疫、27份高抗、28份中抗、42份中感和22份高感大麦材料;在97对SSR引物中挑选出85对多态性较好的引物,85对SSR标记共检测到651个等位变异,平均每个标记为7.57个;SSR标记的基因多样性指数变幅为0.0401～0.8646,平均值为0.5799;多态性信息含量变幅为0.0393～0.8498,平均值为0.5155,137份大麦材料的遗传距离范围为0.1021～0.4807,平均值为0.2774;聚类分析及群体遗传结构分析均将137份大麦种质分为4大类群;根据一般线性模型(GLM,general linear model)共获得7个与大麦抗条纹病显著关联的标记(P＜0.05),解释率在5.80％～17.89％之间,其中标记EBmatc0039的解释率最高;标记EBmac77和MGB357与大麦条纹病抗性呈极显著相关(P＜0.01),二者在一般线性模型中解释率分别为6.07％和9.60％.本研究结果可为大麦抗条纹病育种提供参考.
Abstract
Barley leaf stripe is one of the most serious diseases affecting barley yield and quality.In order to determine the resistant differences of barley germplasm to Pyrenophora graminea and explore candidate markers associated with resistance to Pyrenophora graminea.In this study,we identified the genetic diversity and population structure of 137 barley germplasms by 97 SSR markers and combined them with the results of resistance identification for association analysis.The results showed that 18 accessions were identified to be immunity,27 highly resistant,28 resistant,42 susceptible,and 22 highly susceptible by artificial inoculation with Pyrenophora graminea strain QWC.A total of 651 alleles were detected in 85 primer pairs,with an average of 7.57 alleles.The genetic diversity index ranged from 0.0401 to 0.8646,with an average value of 0.5799.Polymorphism information content ranged from 0.0393 to 0.8498,and the average was 0.5155,the genetic distances ranged from 0.1021 to 0.4807,with an average of 0.2774.The 137 barley germplasms were divided into four subpopulations by Cluster analysis and population structure analysis.A total of seven markers significantly(P＜0.05)were obtainted to be associated with barley resistance to Pyrenophora graminea based on the general linear model,with the explained rates ranging from 5.80％to 17.89％,EBmatc0039 had the highest explained rate.EBmac77 and MGB357 were significantly associated with resistance to barley leaf stripe(P＜0.01),the two markers'rate of phenotypic variation explained were 6.07％and 9.60％in General Linear Model.The results of this study can provide reference for barley resistance to Pyrenophora graminea breeding.
关键词
大麦/大麦条纹病/抗性鉴定/SSR标记/关联分析Key words
barley/Pyrenophoragraminea/resistance identification/SSR markers/association analysis引用本文复制引用
基金项目
国家自然科学基金(32160496)
国家自然科学基金(30771331)
国家大麦青稞产业技术体系(CARS-05-03B-03)
甘南科技计划项目(22CX8NA047)
甘肃省青年科技基金计划(20JR5RA010)
甘肃省教育厅创新基金(2021A-055)
甘肃省教育厅产业支撑计划(2021CYZC-12)
甘肃省省级大学生创新创业训练计划(S202210733005)
出版年
2024
国家科技期刊平台
NSTL
万方数据