Preharvest Sprouting Resistance Test and Functional Markers Assisted Genotyping in Wheat Germplasm Resources
This study aims to identify the pre-harvest sprouting resistance in wheat germplasm resources and molecular markers for marker-assisted selection,ultimately obtaining excellent white grain wheat germplasm resources applicable in wheat breeding.These spikes of 222 wheat germplasm resources were tested for pre-harvest sprouting resistance,and the functional markers of eight anti-pre-harvest sprouting genes,including myb10D,DFR-B,Vp1B3,PM19-A1,MFT-3A,MFT-A2,MKK3-A,QSD1,were used for genotyping.Phenotypic identification results showed that there were significant differences in the relative sprouting percentage of 222 wheat germplasm resources.The relative sprouting percentage ranged from 0 to 1.15,and the average relative sprouting percentage was 0.73.38 wheat materials were identified to be resistant to pre-harvest sprouting,including 9 white wheat,27 red wheat and 2 black wheat.Correlation analysis between allele types and relative sprouting percentage showed that the relative sprouting percentage was significantly correlated with functional markers myb10D,DFR-B,Vp1B3,MFT-3A and MFT-A2,but not with PM19-A1,MKK3-A and QSD1.These results indicated that molecular markers myb10D,DFR-B,Vp1B3,MFT-3A and MFT-A2 could be used for detection of pre-harvest sprouting resistance and marker-assisted breeding.Based on the phenotype and genotype results,nine white wheat germplasm resources with pre-harvest sprouting resistance were selected,including Yunong 914,Yunong 946,Fengdecunmai 30,Fangmai 5,Xumai 029,Lianmai 1901,Baofeng 1903,Zhengmai 829 and 13wang27-8,which could be used for genetic breeding and layout of wheat varieties with pre-harvest sprouting resistance.
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