摘要
为了鉴定小麦种质资源的穗发芽抗性,筛选出有效的抗穗发芽分子标记,进而挖掘优异白粒小麦抗穗发芽种质资源,本研究通过整穗发芽试验对222份小麦种质资源进行穗发芽抗性鉴定,并利用myb10D、DFR-B、Vp1B3、PM19-A1、MFT-3A、MFT-A2、MKK3-A和QSD1等8个抗穗发芽基因的功能分子标记对供试材料进行基因型检测.表型鉴定结果表明,222份小麦种质资源材料的相对穗发芽率存在显著差异,相对穗发芽率变化范围为0～1.15,平均相对穗发芽率为0.73,鉴定出抗穗发芽小麦材料38份,其中包括白粒小麦9份、红粒小麦27份、黑粒小麦2份.等位基因类型与相对穗发芽率相关性分析表明,相对穗发芽率与功能标记myb10D、DFR-B、Vp1B3、MFT-3A和MFT-A2呈极显著相关,而与PM19-A1、MKK3-A和QSD1相关性不显著,说明myb10D、DFR-B、Vp1B3、MFT-3A和MFT-A2等分子标记可用作小麦抗穗发芽基因型检测和分子标记辅助育种.综合表现型和基因型结果,筛选出豫农914、豫农946、丰德存麦30、泛麦5号、徐麦029、连麦1901、保丰1903、郑麦829、13网27-8等9份抗穗发芽白粒小麦种质资源,可用于小麦抗穗发芽遗传育种和抗穗发芽品种布局.
Abstract
This study aims to identify the pre-harvest sprouting resistance in wheat germplasm resources and molecular markers for marker-assisted selection,ultimately obtaining excellent white grain wheat germplasm resources applicable in wheat breeding.These spikes of 222 wheat germplasm resources were tested for pre-harvest sprouting resistance,and the functional markers of eight anti-pre-harvest sprouting genes,including myb10D,DFR-B,Vp1B3,PM19-A1,MFT-3A,MFT-A2,MKK3-A,QSD1,were used for genotyping.Phenotypic identification results showed that there were significant differences in the relative sprouting percentage of 222 wheat germplasm resources.The relative sprouting percentage ranged from 0 to 1.15,and the average relative sprouting percentage was 0.73.38 wheat materials were identified to be resistant to pre-harvest sprouting,including 9 white wheat,27 red wheat and 2 black wheat.Correlation analysis between allele types and relative sprouting percentage showed that the relative sprouting percentage was significantly correlated with functional markers myb10D,DFR-B,Vp1B3,MFT-3A and MFT-A2,but not with PM19-A1,MKK3-A and QSD1.These results indicated that molecular markers myb10D,DFR-B,Vp1B3,MFT-3A and MFT-A2 could be used for detection of pre-harvest sprouting resistance and marker-assisted breeding.Based on the phenotype and genotype results,nine white wheat germplasm resources with pre-harvest sprouting resistance were selected,including Yunong 914,Yunong 946,Fengdecunmai 30,Fangmai 5,Xumai 029,Lianmai 1901,Baofeng 1903,Zhengmai 829 and 13wang27-8,which could be used for genetic breeding and layout of wheat varieties with pre-harvest sprouting resistance.
基金项目
2022年度河南省重大科技专项(221100110700)
河南省农业良种联合攻关项目(2022010102)
河南省现代农业产业技术体系建设(HARS-22-01-G2)
河南省科技研发计划联合基金(222103810002)
2022年度河南省产业研发联合基金重大项目(225101610048)
河南农业大学拔尖人才项目(30500678)
国家科技期刊平台
NSTL
万方数据