分子标记是遗传研究的基础工具,广泛应用于遗传多样性研究、种质鉴定、遗传图谱构建和基因定位等领域.本研究利用Barley SNP 50K芯片对遗传背景来源广泛的大麦核心资源进行SNP检测,筛选出一系列多态性高的SNP位点,并开发出124个KASP分子标记.利用43份不同地理来源的大麦种质检测其有效性,初步筛选出56个KASP标记;以最小等位基因频率≥0.40、多态信息含量≥0.45为标准挑选18个高质量的KASP标记并用于绘制98份江苏省大麦品种的系统发育树,结果显示这18个KASP标记能够将具有相同地理来源及亲缘关系近的大麦材料聚为一类,表明上述KASP标记在大麦品种鉴定、大麦资源亲缘关系分析以及群体划分方面具有一定的指导意义和应用价值.同时构建了 98份江苏大麦品种的SNP指纹图谱,验证了 KASP技术在我国大麦品种鉴定中的可行性.开发的KASP标记能够准确、快速鉴定大麦品种,有助于大麦种质资源的科学规范管理和遗传多样性研究.
Development and Application of High Efficiency KASP Markers in Barley Based on High Throughput Chip
Molecular markers are the basic tools of genetic research,which are widely used in genetic diversity research,germplasm identification,genetic map construction and gene mapping.In this study,Barley SNP 50K chip was used to detect SNP in barley core resources with wide genetic background,and a series of dimorphic SNPs with high polymorphism were selected,and 124 KASP markers were developed.43 barley germplasms from different geographical sources were used to test their effectiveness,and 56 KASP markers were initially screened out.Eighteen high-quality KASP markers were selected based on MAF(minor allele frequency)≥0.40 and PIC(polymorphic information content)≥0.45 criteria and applied to construct phylogenetic trees of 98 Jiangsu barley varieties.The results showed that barley materials with the same geographical origin and close relatives could be grouped into one class.These results indicate that KASP markers have guiding significance and application value in barley variety identification genetic relationship analysis of barley resources and population division.At the same time,the SNP fingerprint of 98 Jiangsu barley varieties was constructed,which verified the feasibility of KASP technique in barley varieties identification in China.The developed KASP marker can identify barley varieties accurately and quickly,which is helpful to the scientific and standardized management of barley germplasm resources and the study of genetic diversity.
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