小麦B-ARR转录因子家族基因鉴定与表达模式分析
Genome Identification and Expression Analysis of B-ARR Transcription Factors Family in Wheat
王长彪 1尹誉蓉 2程泽 2任永康 2牛瑜琦 2刘江 3韩斌 3杨晟 2唐朝晖2
作者信息
- 1. 山西农业大学农学院,太原 030031;山西农业大学生命科学学院,太原 030031
- 2. 山西农业大学农学院,太原 030031
- 3. 山西农业大学生命科学学院,太原 030031
- 折叠
摘要
B型反应调节因子(B-ARR,type-B authentic response regulator)家族基因是细胞分裂素信号转导的正向调节因子,在植物生长发育和抗非生物胁迫中起重要作用.然而,目前对小麦中B-ARR基因家族的研究还很少.本研究从小麦基因组中鉴定出25个B-ARR基因家族成员,利用生物信息学方法分析其理化性质、基因结构、顺式作用元件和非生物胁迫诱导的表达模式.结果表明,B-ARR蛋白都定位于细胞核中,B-ARR蛋白二级结构以α螺旋和无规则卷曲为主要结构.B-ARR基因在小麦染色体上分布不均匀,其中7号染色体上含有的B-ARR基因数量最多.此外,在B-ARR的启动子区域中鉴定出了与生长发育、激素响应以及生物和非生物胁迫相关的多种顺式作用调节元件.RT-qPCR分析表明,基因TaARRM-like9、TaARRM-like10、TaARRM-like12和TaARRM-like13在干旱、盐和低温胁迫下表达量显著上调,能够响应非生物胁迫.本研究为进一步研究B-ARR转录因子在小麦发育和非生物胁迫应答中的作用奠定了基础.
Abstract
The type-B authentic response regulator(B-ARR)family members are positive regulators in cytokinin signal transduction,and play important roles in plant growth and development and resistance to abiotic stresses.However,there are few studies on the B-ARR gene family in wheat.In this study,25 B-ARR gene family members were identified from wheat genome,and their physicochemical properties,gene structure,cis-acting elements and abiotic stress-induced expression patterns were analyzed by bioinformatics methods.The results showed that all B-ARR proteins were localized in the nucleus based on bioinformatics prediction,and their secondary structure was mainly consisting of α-helix and random crimp.B-ARR genes were not evenly distributed on wheat chromosomes,and the number of B-ARR genes was the highest on chromosome 7.In addition,multiple cis-acting regulatory elements related to growth and development,hormone response,and biological and abiotic stress have been identified in the promoter regions.RT-qPCR analysis showed that the relative expression of TaARRM-like9,TaARRM-like10,TaARRM-like12 and TaARRM-like13 were significantly up-regulated under abiotic stresses treatments,including drought,salt and low temperature.This study laid a foundation for further research on the role of B-ARR transcription factor in wheat development and abiotic stress response.
关键词
小麦/B-ARR转录因子/生物信息学分析/非生物胁迫/表达分析Key words
wheat/B-ARR gene family/bioinformatics analysis/abiotic stress/expression analysis引用本文复制引用
基金项目
国家重点研发计划项目(2022YFD1200901)
山西省重点研发计划项目(202102140601001)
山西省现代农业产业技术体系建设专项资金(2023CYJSTX02-06)
出版年
2024
国家科技期刊平台
NSTL
万方数据