Genome Identification and Expression Analysis of B-ARR Transcription Factors Family in Wheat
The type-B authentic response regulator(B-ARR)family members are positive regulators in cytokinin signal transduction,and play important roles in plant growth and development and resistance to abiotic stresses.However,there are few studies on the B-ARR gene family in wheat.In this study,25 B-ARR gene family members were identified from wheat genome,and their physicochemical properties,gene structure,cis-acting elements and abiotic stress-induced expression patterns were analyzed by bioinformatics methods.The results showed that all B-ARR proteins were localized in the nucleus based on bioinformatics prediction,and their secondary structure was mainly consisting of α-helix and random crimp.B-ARR genes were not evenly distributed on wheat chromosomes,and the number of B-ARR genes was the highest on chromosome 7.In addition,multiple cis-acting regulatory elements related to growth and development,hormone response,and biological and abiotic stress have been identified in the promoter regions.RT-qPCR analysis showed that the relative expression of TaARRM-like9,TaARRM-like10,TaARRM-like12 and TaARRM-like13 were significantly up-regulated under abiotic stresses treatments,including drought,salt and low temperature.This study laid a foundation for further research on the role of B-ARR transcription factor in wheat development and abiotic stress response.
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