Evaluation of Cross Resistance to ALS Herbicides and Expression Analysis of Resistance Gene BnAHAS1-3R in Rapeseed M196
In order to identify effects of the cross-resistance and the function of resistant genes BnAHAS1-3R in germplasm M196,this study examined the resistance effect of rapeseed M196 and N131(wild type)by spraying sulfonylurea,sulfonlyaminocarbonyl-triazolinone,imidazolinone,triazolopyrimidine,and pyrimidyl-benzoate with different concentration gradients(0,1/4,1/2,1,2 and 4 times the recommended concentration of herbicides in the field),respectively,at 3-5 leaf stage.Based on the greenhouse and field results,the cross-resistance of SU and SCT was observed simultaneously to be the strongest,while IMI,TP and PB were lower.Results of qRT-PCR and in vitro enzyme inhibition experiments in transgenic Arabidopsis indicated that the cross-resistance of ALS herbicides was caused by the elevated expression of the mutated allele of BnAHASl-3R and the decrease on enzyme sensitivity to herbicides.The study provides a theoretical basis for preventing and resolving the potential problems about weed resistance and drift of resistance gene that may arise from the widespread promotion of resistant varieties.
Brassica napus L.herbicide resistant new germplasmcross-resistancefunction analysis
NETL
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