Analysis of the Microecological Factors of Target Leaf Spot of Tobacco Variety'K326'in Chongqing Tobacco Area
In order to determine the key factors and microbial structure of the leaf microenviron-ment of tobacco target spot disease in'K326'tobacco variety in Chongqing tobacco region,'K326'tobacco variety was taken as experimental materials.Samples of diseased and healthy leaves of tobacco plants were collected in Youyang County,Chongqing at 40 d,60 d,80 d and 100 d after transplanting.The bacterial community structure in the leaves was analyzed by high-throughput sequencing method,and the climatic factors and disease occurrence during the growth period of tobacco plants were analyzed.At 40 days after transplantation,the onset of target spot disease was observed,exhibiting an upward trend in both disease incidence and se-verity index,and the period between 60 and 80 days after transplantation was identified as the peak phase of disease incidence.The dominant bacteria in the diseased leaf boundary were Pro-teobacteria and Thick-walled Bacteria.The dominant bacteria were Panbacterial,Pseudo-monas,Microbacterium,etc.The dominant phyla of the healthy leaf boundary bacteria were:Thickwallida,Cyanophyta,Actinomyceta,Bacteroides,etc.The dominant genera were Pseud-omonas,Acinetobacter,Microbacterium,Bacillus,Pantobacterium etc.The overall communi-ty richness of healthy leaves was greater than that of diseased leaves.The bacterial community structure of diseased leaves and healthy leaves collected on the 40,60 and 100 days after trans-planting was significant different.In the case of severe target spot disease,there were signifi-cant differences in the bacterial community structure between the diseased leaves and the health-y leaves.
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