Cloning and Sequencing Analysis of the Readthrough Protein Gene of Different Tobacco Vein Distorting Virus Isolates of Yunnan Province
In order to explore the variationsinthe readthrough protein gene(rtp gene)sequence-samong isolates of tobacco vein distorting virus(TVDV)collected at different times and from various regions of Yunnan Province,and analyze the factors contributing to the recent decelera-tion of tobacco bush top diseasein the field,the rtp gene sequences of TVDV was cloned and an-alyzed from the diseased tobacco samples collected from Chuxiong,Dali,Baoshan and Hong-heAutonomous Prefectures/City in Yunnan Province from 2020 to 2022.The rtp gene se-quences of seven different TVDV isolates were obtained,all of them were 2 172 nucleotide(nt)in length,consisting of a 618-ntcp gene and 1 554-ntrtd domain.Consequently,the TVDV RTP protein has a total of 722 amino acids(aa),comprising 205 aa of CP protein and 517 aa of RTD.Multiple alignment analysis results showed that the nt sequence identities of the cp genebe-tween the seven TVDV isolates obtained in this study and the TVDV isolates registered in Gen-Bank were 98.71％～100％.Similarly,the aa sequence identities of the CP protein among differ-ent TVDV isolates were 98.05％～100％,with four aavariations.The nt sequence identities of the rtddomain between the seven TVDV isolates obtained in this study and the TVDV isolates derived fromGenBankranged from 96.68％to 99.81％.Likewise,the aa sequence identities of their RTD domain were 96.52％～100％,with thirty-nine aa differences.The variation of rtp gene in these TVDV isolates may affect the efficiency of aphid transmission of TVDV,and thus affect the occurrence and prevalence of tobacco bush top disease in the field.
tobacco vein distorting virustobacco bushy top diseasereadthrough proteinmo-lecular variationaphid transmission
万方数据
维普
