Screening andIdentification of Biocontrol Endophytic Fungi from Hippophae rhamnoides L.and Analysis of Their Cellulase Activity
In order to screen biocontrol endophytic fungi from Hippophae rhamnoides L.,endo-phytic fungi were isolated from healthy H.rhamnoides L.leaves and fruits by tissue homogeni-zation method.The strains with antagonistic activity were screened,identified by morphology and molecular biology,and the cellulase activity of the active strains was analyzed.A total of 16 strains with good inhibitory effects on 8 plant pathogenic fungi was screened from 97 endophytic fungi,among which strains SJ-83 and SJ-116 had the best inhibitory effects on 8 pathogens.The morphological characteristics of 16 strains of endophytic fungi were observed.It was found that strain SJ-93 produced brown conidia with transverse and longitudinal membrane,which was i-dentified as Alternaria.The strain SJ-83 had flocculent,light green-brown mycelium,and pro-duced brown transversely septate conidia,which was a fungus of the genus Bipolaris.The cel-lulase activity was determined on Congo red medium by transparent circle method.The results showed that the cellulase activity of strain SJ-116 was the highest,and the radius of transparent circle was 1.6 cm.The results of this study provided basic materials for the subsequent develop-ment of biocontrol endophytic fungi for H.rhamnoides L.
万方数据
维普
