Study on Optimal Solid Fermentation Conditions of Trichoderma harzianum TMN-1
Trichoderma harzianum TMN-1 has good control effect on tobacco bacterial wilt.In order to widely apply it to control the field disease,it is necessary to prepare its bactericidal a-gent.Therefore,this study mainly explored the solid fermentation conditions of T.harzianum TMN-1 strain,a biocontrol bacterium isolated from soil,under laboratory conditions.Based on the determination of fermentation solid medium,fermentation temperature,water content,in-oculation amount,and external nutrient elements,the study explored the influence of different conditions on the conidial production of TMN-1 strain.The results showed that rice husk pow-der had the best effect among the fermentation conditions of T.harzianum and could be used as a good solid fermentation medium for T.harzianum TMN-1 strain.Meanwhile,the optimum fermentation conditions for T.harzianum TMN-1 strain were as follows:8％of inoculation a-mount,30％of water content,and 28 ℃ of fermentation temperature.Under this condition,the conidia yield of T.harzianum could reach 1 × 110 cfu/g for one week.At the same time,external starch as carbon source can significantly increase the conidium production of T.harzia-num TMN-1.The research can provide basic conditions and theoretical basis for the preparation of T.harzianum solid bactericidal agent and the commercialization of T.harzianum.
Trichoderma harzianumsolid fermentationbiological control
万方数据
维普
