Pathogenic Identification of Peach Tree Fungal Diseases in Chongqing
Peach tree is an important economic tree species in China.In recent years,with the in-crease of planting area,occurrence of the various diseases have had an impact on the yield and quality of peaches in China,among which fungal diseases are particularly serious.Field observa-tions revealed that the main diseased parts of peach trees in Beibei District,Chongqing are the leaves and fruits.Pathogens infect the leaves,causing the formation of disease spots and irregu-lar perforation on the leaf surface,and infect the fruits to cause partial or complete rot of the fruit.This study collected the diseased peach tree samples from orchards in Beibei District,Chongqing.Through fungal isolation and purification,10 different species of fungal strains were obtained,and the corresponding pathogenic strains were determined by using Koch's rule.Fun-gal DNA was extracted and analyzed with multi gene sequences to amplify ITS,CAL,GAP-DH,and TUB2 gene fragments and construct the phylogenetic tree.Combined with morpho-logical characteristics,the fungus causing peach tree leaf perforation disease was ultimately de-termined to be Colletotrichum gloeosporioides,and the fungus causing fruit rot disease was de-termined to be Monilinia fruticola.The research results can provide theoretical support for the subsequent prevention and control of fungal diseases in peach trees.
Prunus persicafungal diseasespathogen identificationpathogenicity test
万方数据
维普
