Biological Characteristics of Amomum tsao-ko Pseudostem Black Spot Disease Pathogen Pestalotiopsis microspora
To elucidate the biological characteristics of Amomum tsao-ko black spot disease fun-gus CGJ-3(Pestalotiopsis microspora),the growth rate method and hemocytometer method were used to assess the effects of carbon sources,nitrogen sources,temperature,light,and pH on mycelial growth and spore production.The results showed that CGJ-3 could grow and pro-duce spores on media containing glucose,mannitol,sorbitol,fructose,sucrose,maltose,starch,and xylose,with glucose being the most suitable carbon source for mycelial growth and fructose as the best for spore production.Except urea,the fungus grew and produced spores on media with beef extract,peptone,glycine,potassium nitrate,sodium nitrate,ammonium phosphate,ammonium nitrate,methionine,and threonine as nitrogen sources,with beef ex-tract being the optimal nitrogen source.The suitable growth temperature for the pathogen was 23～29 ℃,with an optimum temperature at 27 ℃;the suitable temperature range for spore production was 25～27 ℃,with the optimum temperature also at 27 ℃.Light promoted the growth and spore production of the pathogen,while planting evergreen broadleaf trees with A.tsao-ko plants could inhibit the reproduction of the pathogen.The fungus was able to grow at a pH range of 4～12 and produced spores at a pH of 4～11,with the optimal pH for growth be-ing 6 and the optimal pH for spore production being 7.The application of procymidone,pyra-clostrobin,and chlorothalonil can control the A.tsao-ko black spot diseases caused by the genus Pestalotiopsis.The results provide theoretical support for the planting of evergreen broadleaf trees and the application of urea to inhibit the growth and spore production of the pathogen.
Pestalotiopsis microsporaPseudostem Black Spot DiseaseAmomum tsao-koco-nidial productionprochloraz
万方数据
维普
