Isolation and Identification of the Pathogen Causing Anthracnose in Passion Fruitin Guangdong
With the development of passion fruit industry in China,diseases and pests have be-come the problem that cannot be ignored in the production of passion fruit.In this paper,patho-gens in the anthracnose disease infected samples from Heping County,Heyuan City and Nansha District,Guangzhou City,Guangdong Province were isolated and purified by tissue separation method,and 4 high-frequency strains were obtained.Through pathogenicity testing,it was con-firmed that the strain ZB2-1 and strain DGZB could infect the fruit and branch of passion fruit,respectively.The morphological characteristics of the two strains were similar to those of Colle-totrichum brasiliense.By constructing a multigene combined phylogenetic tree(ITS-TUB2-ACT-CHS1-HIS3-GAPDH),it was found that the two strains were in the same branch with C.brasiliense,with a 100％bootstrap support value.The pathogen identification was carried out by combining morphological characteristics,pathogenicity and phylogenetic tree construction.The results indicated that C.brasiliense was the pathogen of anthracnose infecting passion fruits and branches in Heping County,Heyuan City and Nansha District,Guangzhou City.
万方数据
维普
