Effect of Melaleuca alternifolia Cheel and Its Volatile Compounds on Behavior of Diaphorina citri Kuwayama and Its Application
The Diaphorina citri Kuwayama is an insect pest,which damages the young shoots of citrus and serves as a vector for the transmission of citrus Huanglongbing.Field investiga-tions indicated that citrus orchard with protective belts of Melaleuca alternifolia Cheel,could effectively control the Diaphorina citri Kuwayama.The volatile components of M.alternifolia from citrus orchards in Deqing City,Guangdong Province were analyzed using gas chromatogra-phy-mass spectrometry(GC-MS).Combined with gas chromatography-electroantennographic detection(GC-EAD)technology,the antennal responses of Diaphorina citri Kuwayama adults to the essential oil of M.alternifolia were also studied.The olfactory behavioral responses of Asian citrus psyllid to M.alternifolia plant,plant essential oil,and its major volatile com-pounds were tested by Y-tube olfactometer.The results showed that bio-type of M.alternifolia was 4-terpineol,and its volatile compounds contained 29 components.The antennae of female Diaphorina citri Kuwayama exhibited electrophysiological responses to α-terpinene,γ-ter-pinene,and 4-terpineol of M.alternifolia essential oil,while the antennae of male psyllids only responded to γ-terpinene and 4-terpineol.Behavioral response tests indicated that adult Diapho-rina citri Kuwayama exhibited significant avoidance to branches and leaves of M.alternifolia,as well as to 20 μL/mL and 30 µL/mL of M.alternifolia essential oil and 100 μl/ml 4-terpine-ol.However,they were significant attracted by 0.1 µL/mL γ-terpinene.The relationship be-tween the Diaphorina citri Kuwayama and M.alternifolia was deeply explored.The study laid a foundation for utilizing the 4-terpineol-type M.alternifolia essential oil to control Diaphori-na citri Kuwayama,offering insights for the development of environmentally friendly pest con-trol techniques for this pest.
万方数据
维普
