Regulatory Effect of CA72-4 on Monocyte IL-1β and Acute Gout Attack
Objective:To investigate the regulatory effect of carbohydrate antigen 72-4(CA72-4)on monocyte interleukin 1β(IL-1β)and acute gout attack.Method:The peripheral plasma of acute gout patients who admitted to the Gout Specialist Clinic of Zibo Central Hospital was collected,and the content of CA72-4 was analyzed to obtain the plasma of patients with CA72-4≥50 U/mL,which was randomly divided into no-CA72-4 group(n=37)and CA72-4 group(n=38),no-CA72-4 group used DynaMag magnetic frame to remove CA72-4 from the plasma,while CA72-4 group was not treated,THP1 cells were induced by plasma before and after treatment,the induced THP1 cell culture medium and cells were collected,and the expression level of IL-1β in THP1 cells and culture medium was detected by enzyme-linked immunoassay(ELISA).Subcutaneous injection of sterilized and filtered air into the back of mice resulted in subcutaneous air sac,and then monosodium urate(MSU)crystals were injected into the air sac to construct gouty synovitis model,the model mice were divided into the experimental group(n=10)and the control group(n=10),the experimental group was intraperitoneally injected with 1.5×1010 PFU/mL overexpressed adenovirus(Ad)-CA72-4,while the control group was injected with adenovirus Ad-ctr,plasma and synovial fluid of mice were collected,and CA72-4 detection kit was used to detect CA72-in the plasma and synovial fluid of mice.The expression levels of IL-1β and tumor necrosis factor-α(TNF-α)in plasma of mice were detected by ELISA,the gene and protein expression levels of IL-1β and nucleotide binding oligomerization domain-like receptor protein 3(NLRP3)inflammasome in plasma of mice in each group were detected by real-time quantitative fluorescence polymerase chain reaction and Western blot.Result:Compared with CA72-4 group,IL-1β in THP1 cells and culture medium in no-CA72-4 group were significantly increased,and the differences were statistically significant(P<0.001);compared with the control group,the expression levels of CA72-4 in plasma and synovial fluid of mice in the experimental group were significantly increased,and the differences were statistically significant(P<0.001);compared with the control group,the expressions of IL-1β and TNF-α in the plasma of the experimental group were significantly increased,and the differences were statistically significant(P<0.001);compared with the control group,the gene and protein expressions of IL-1β and NALP3 in the plasma of mice in the experimental group were significantly increased,and the differences were statistically significant(P<0.001).Conclusion:CA72-4 can enhance the secretion of inflammatory factors in monocytes and aggravate the inflammatory response of gout,and its specific mechanism needs further study.
国家科技期刊平台
NSTL
万方数据
