Analysis of Genotyping Detection of HPV in Patients with Cervical Lesions and Its Clinical Significance
Objective:To analyze the genotyping detection of HPV in patients with cervical lesions and its clinical significance.Method:The clinical data of 125 patients with cervical lesions who admitted to Taixing People's Hospital from May 2021 to May 2023 were retrospectively analyzed,and HPV genotyping was performed in all of them.The positive rate of HPV infection and HPV genotyping of 125 patients with cervical lesions were analyzed(multiple infection repeated counting).The positive rate of HPV infection and high-risk HPV infection in patients with different degree of cervical lesions were compared.Using pathological examination as the"gold standard",the accuracy,sensitivity and specificity of liquid based thin layer cytology(TCT)alone and TCT combined with HPV genotyping in the diagnosis of cervical cancer were analyzed and compared.Result:Among 125 patients with cervical lesions,the positive rate of HPV infection was 51.20%(64/125),and the total number of HPV genotypes in HPV positive patients was 92,of which 63.04%(58/92)were high-risk HPV and 36.96%(34/92)were low-risk HPV.The top 5 genotypes with the highest positive rate of high-risk HPV infection were HPV16(16.30%),HPV18(11.96%),HPV58(8.70%),HPV53(6.52%),HPV51(4.35%);the top 3 genotypes with the highest positive rate of low-risk HPV infection were HPV6(8.70%),HPV11(7.61%)and HPV40(4.35%),respectively.There were significant differences in the positive rates of HPV infection and high-risk HPV infection in patients with different levels of cervical lesions(P＜0.05).The accuracy of TCT alone was 78.40%(98/125),the sensitivity was 66.67%(18/27),the specificity was 81.63%(80/98),the positive predictive value was 50.00%(18/36),the negative predictive value was 89.89%(80/89),and the Kappa value was 0.431;the accuracy of TCT combined with HPV genotyping was 88.00%(110/125),the sensitivity was 59.26%(16/27),the specificity was 95.92%(94/98),the positive predictive value was 80.00%(16/20),and the negative predictive value was 89.52%(94/105),the Kappa value was 0.609.The accuracy and specificity of TCT combined with HPV genotyping in the diagnosis of cervical cancer were significantly higher than those of TCT alone,the differences were statistically significant(P＜0.05);the sensitivity of TCT combined with HPV genotyping in the diagnosis of cervical cancer was lower than that of TCT alone,but the difference was not statistically significant(P＞0.05).Conclusion:The types of cervical lesions are closely related to HPV genotypes,and there are significant differences in the positive rate of HPV infection and high-risk HPV infection in patients with different malignant degrees of cervical lesions,therefore,HPV genotyping is of great significance in assisting cervical cancer screening.
Cervical lesionsHuman papillomavirusGenotypingCervical cancer
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