Effect of Different Nucleic Acid Extraction Methods in Detection of Epstein-Barr Virus and Human Cytomegalovirus
Objective:To evaluate the performance and clinical application value of three different nucleic acid extraction methods in nucleic acid detection of Epstein-Barr virus(EBV)and human cytomegalovirus(HCMV).Method:Nucleic acids were extracted by manual column extraction method,manual magnetic bead method and automatic magnetic bead method.quantitative real-time polymerase chain reaction(qRT-PCR)was used to determine the DNA of EBV and HCMV.Evaluate detection precision,linear range,and minimum detection limit.Thirty-five clinical samples from the Fourth Affiliated Hospital of Soochow University in December 2023 were selected for clinical application evaluation,and the linear relationship between manual magnetic bead method and automatic magnetic bead method for nucleic acid extraction was evaluated.Result:The CV values of three methods in terms of precision were all less than 5%,and there was a good linear relationship in the sample concentration of 102-107 copies/mL,the verification results of the minimum detection limit were also qualified;in the evaluation of clinical application,the positive rate of manual column extraction method was higher than those of the other two methods,but the difference was not statistically significant(P>0.05).There was a good correlation between manual magnetic bead method and automatic magnetic bead method in the detection of EBV and HCMV samples.Conclusion:The quantitative detection results of EBV and HCMV samples by three nucleic acid extraction methods all meet the requirements,the appropriate extraction method can be selected according to the detection requirements of different sample sizes to reduce the experimental cost and improve the detection efficiency.
万方数据
维普
