Using the 3512 optimum regression design with three factors and five levels, effects of applications of Cu, Zn and Se on contents of main active constituents (total flavonoid and chlorogenic acids) and selenium in the flower of Chrysanthemum morifolium were investigated by a pot experiment. The results show that applying Cu, Zn and Se with enough other macro and medium and trace elements is able to increase contents of total flavonoid and chlorogenic acids in the flower of Chrysanthemum morifolium significantly, and the interaction of the three elements also reacts to the influ-ence on the active constituents: contents of total flavonoid and chlorogenic acids are increased significantly under the Cu -Se interaction; however, the contents are decreased under the Cu-Zn and Zn-Se interactions. Higher concentrations of Cu have inhibition or even toxicity effects on the crop growth and the main active constituents. The formation and accu-mulation of the two main active constituents are restrained under Zn deficiency, and Zn is a dominant nutrient factor of af-fecting quality of Chrysanthemum morifolium. There is significant positive correlation between Se content of the flowers and Se application amount. The selenium content of the flowers is not able to be influenced by the application of Cu and Zn. This result provides theoretical basis for developing Se enriched Chrysanthemum morifolium Ramat and its series products.
Chrysanthemum morifolium Ramat.combined application of Cu, Zn and Setotal flavonoidehlorogenic acidSe content