Effects of NaCl stress on growth and physiological-biochemical indexes of Iris lactea var.chinensis
Taking 1/2Hoagland solution as basic culture solution, effects of 1,2, 3, 4, 6, 8 and 10 g · L-1 NaCl stress on some growth and physiological-biochemical indexes of Iris lactea Pall. var.chinensis (Fisch.) Koidz. seedling were researched by hydroponic culture method. The results show that with rising of NaCl concentration, height and root length, dry weights of above-ground part, root and whole plant of Ⅰ. lactea var. chinensis seedling decrease gradually, but height and dry weights of aboveground part and whole plant are significantly higher than those of the control under 1 g · L-1 NaCl stress.With rising of NaCl concentration and prolonging of stress time, relative conductivity and MDA content in leaf of Ⅰ. lactea var. chinensis seedling appear the trend of increasing gradually. In which, relative conductivity increases slightly but has no significant difference with that of the control under 1-4 g · L-1 NaCl stress for 7 or 14 d, and also, MDA content has no significant difference with that of the control under 1-8 g · L-1 NsCl stress for 7 d. Contents of chlorophyll and proline and SOD activity in leaf appear the trend of increasing firstly and then decreasing, and these indexes reach the highest values under NaCl stress for 14 or 21 d. With rising of NaCl concentration, proline content and SOD activity increase continuously and generally are significantly higher than those of the control. Chlorophyll content appears the trend of increasing under low concentration and decreasing under high concentration with rising of NaCl concentration, in which, that is the highest of treatment groups with 3 and 4 g · L-1 NaCl. Soluble protein content of every group under NaCl stress is not significantly different with that of the control at the 7th or 14th day, but that appears the trend of increasing under low concentration and decreasing under high concentration at the 21st day with rising of NaCl concentration, while that decreases gradually at the 28th day with rising of NaCl concentration. Overall, NaCl stress with low concentration and short time has an un-obvious inhibition effect or even a slight promotion effect on growth and metabolism of Ⅰ. lactea var. chinensis seedling, while the inhibition effect of NaCl stress with high concentration and long time is obvious. As survival of all treated seedlings after the experiment finished, and combining with determination results of growth and physiological-biochemical indexes, it is preliminarily concluded that Ⅰ. lactea var. chinensis has a stronger resistance to NaCl stress.
Iris lactea Pall. var. chinensis (Fisch.) Koidz.NaCl stressgrowth indexphysiological-biochemical index
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