Analyses on morphology and firmness and observations on anatomical structure and micromorphological characteristics of fruit of different cultivars of blackberry (Rubus spp.)
Taking mature fruits of three cultivars ‘ Arapaho',‘ Boysenberry' and ‘ Kiowa' of blackberry (Rubus spp.) as the experimental materials,morphological indexes and firmness of fruits were determined,and anatomical structure and micro-morphological characteristics of epicarp and pulp were observed by paraffin section and SEM techniques.And on these bases,relationship between fruit firmness and fruit structure was also discussed.The results show that fruit firmness value of cultivar ‘ Arapaho' (0.79 lb · mm-2) is bigger than that of cultivars ‘ Boysenberry' and ‘ Kiowa’ (both 0 lb · mm-2).And fruit longitudinal and transverse diameters and weight per fruit of cultivar ‘Arapaho' are extremely significant less than those of cultivars ‘ Boysenberry' and ‘ Kiowa'.The observation result of paraffin section shows that epicarp of three cultivars is thinner and without cuticle covering,consists of 1-2 layers of epidermal cells.In which,epicarp of cultivar ‘ Arapaho' consists of one layer of short and small epidermal cells arranged densely,that of cultivar ‘ Boysenberry' consists of two layers of long and thin epidermal cells arranged orderly and loosely,and that of cultivar ‘ Kiowa' consists of two layers of epidermal cells with fold cell wall and local cavity in fruit surface.Mesocarp of cultivar ‘ Arapaho'consists of a lot of relatively complete parenchyma cells and contains vascular bundles without disintegration,while,that of cultivars ‘Boysenberry' and ‘Kiowa' are uniformly distributed by disintegration parenchyma cells.The observation result of SEM show that epicarp of cultivar ‘Boysenberry' possesses sinuolate ornamentation,irregular epidermal cells,and sparse trichomes and lamellar exudates; that of cultivar ‘ Kiowa' possesses obviously irregular sinuosity ornamentation,while that of cultivar ‘ Arapaho' has compact and dense texture,and cell outline clear and shape regular of epidermal cells.Pulp cells of three cultivars disintegrates in different degrees,but there are large gelatinous substances without disintegrating in pulp cell of cultivar ‘Arapaho' According to these observation results,it is conjectured that there is a certain relationship between anatomical structure and micro-morphological characteristics of pericarp and pulp of blackberry fruit with its firmness.