Observation on pollen character and selection of pollen culture and storage conditions of three bamboo species
Pollen character and viability of Shibataea chinensis Nakai,Bambusa multiplex (Lour.) Raeusch.ex Schult.et J.H.Schult.and Arundinaria simonii f.heterophyllus (Makino et Shirasawa) Muro were observed,and suitable culture and storage conditions of pollen were selected by taking pollen germination rate as the index.Results show that pollen of three bamboo species is nearly spherical with a single aperture,obvious pore ring and cover,aperture is concave.The highest germination rate of S.chinensis,B.multiplex and A.simonii f.heterophyllus is 9.1％,15.8％ and 76.5％,respectively.Suitable culture medium for pollens of S.chinensis and B.multiplex contains boric acid and sucrose with mass ratios of 0.001％ and 15％,respectively,while that for pollen of A.simonii f.heterophyllus contains boric acid and sucrose with mass ratios of 0.001％ and 20％,respectively,and the appropriate culture time is less than 3 h.The suitable storage conditions for pollen of A.simonii f.heterophyllus are storing fresh pollen under 4 C or-5 ℃ for less than 10 h.
bamboopollen morphologypollen viabilitypollen germination rateculture and storage conditions
NETL
NSTL
万方数据
维普
