Effect of nitrogen with different forms and ratios on growth, and content and total amount of ;nitrogen component in root of Isatis indigotica
Taking five provenances of Isatis indigotica Fort. from Bozhou of Anhui, Zhangye of Gansu, Fuyang of Anhui, Yuncheng of Shanxi and Shangluo of Shaanxi as experiment materials, differences in fresh and dry weights of root per plant, root drying rate, contents of free total amino acids, soluble protein and total nitrogen in root, and total amounts of free total amino acids, soluble protein and total nitrogen in root per plant of different provenances of I. indigotica under conditions of without applying nitrogen (CK),only applying NO3--N (T1) or NH4+-N (T2),mixed applying nitrogen〔n(NH4+-N):n(NO3--N)=75:25 (T3), n(NH4+-N):n(NO3--N)=50:50 (T4) and n(NH4+-N):n(NO3--N)=25:75 ( T5 )〕, and only applying CO ( NH2 ) 2 ( T6 ) were compared and analyzed by field experiment method. The results show that under same nitrogen applying condition, there is a certain difference in various indexes in root of I. indigotica from different provenances. While, compared with their own control, there is also a certain difference in various indexes in root of I. indigotica from the same provenance under different nitrogen applying conditions. In general, root growth indexes of I. indigotica from Bozhou of Anhui and Yuncheng of Shanxi provenances are all higher in T6 group, those from Zhangye of Gansu provenance are relatively higher in T2 group, those from Fuyang of Anhui provenance are the highest in T5 group, and those from Shangluo of Shaanxi provenance are higher in T4 group. Under different nitrogen applying conditions, total amounts of free total amino acids, soluble protein and total nitrogen in root per plant of I. indigotica from Bozhou of Anhui provenance are all lower than those of its control, and the three indexes are relatively higher in T6 group. The above three indexes in root from Zhangye of Gansu and Shangluo of Shaanxi provenances are the highest in T2 and T3 groups, respectively, while those from Fuyang of Anhui and Yuncheng of Shanxi provenances are higher in T5 and T1 groups, respectively. It is suggested that there is difference among provenances in effect of nitrogen with different forms and ratios on root growth and root nitrogen component accumulation of I. indigotica, and nitrogen form and ratio beneficial to growth and nitrogen component accumulation of root from the same provenance are also different. Therefore, in order to obtain high yield and good quality indigowoad root medicinal materials, it is suggested to take appropriate nitrogen applying measure for different provenances during cultivation process, and take into account growth amount and effective component content.
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NSTL
万方数据
维普
