Dynamic analysis on appearance and morphology,nutrients,and gene expression during the development process of blackberry(Rubus spp.)fruits
To clarify the dynamic variation pattern of appearance and morphology,nutrients,and gene expression during the development process of blackberry(Rubus spp.)fruits,the blackberry cultivar'Arapaho'was taken as the experimental material,and the phenotypic indexes,physiological and biochemical indexes,and gene expression levels of fruits at different developmental stages were compared and analyzed.The results show that during the development process of blackberry fruits,the fruits turn from cyan to dark purple,the red and green phase value(a*)of the fruits at S1(more than 95％of the fruits are cyan)stage and the yellow and blue phase values(b*)of the fruits at S4(about 50％of the fruits turn dark purple)and S5(more than 95％of the fruits are dark purple)stages are negative,the fruit lightness(L*)and color saturation(C*)of the fruits at S1 stage are the highest,while those at S5 stage are the lowest;hardness and titratable acid content show a tendency to decrease,horizontal diameter,vertical diameter,mass per fruit,soluble solid content,pH value,and anthocyanin content show a tendency to increase in general,and total phenols content and flavonoid content show a tendency to first decrease and then increase.The cumulative contribution rate of the first two principal components in principal component analysis reaches 87.4％,and pH value,titratable acid content,and a*value are the main evaluation indexes of fruit development dynamics.The correlation analysis result shows that the phenotypic indexes of fruits mostly show significant or extremely significant correlations with physiological and biochemical indexes.Titratable acid content shows significant or extremely significant correlations with total phenols content,flavonoid content,and anthocyanin content,speculating titrable acid content can affect the contents of total phenols,flavonoid and anthocyanin in blackberry fruits.The result of real-time fluorescent quantitative PCR result shows that RuSUS and RuFRK may positively regulate sugar metabolism in blackberry fruits,RuACO and RuIDH may negatively regulate the accumulation of organic acids in the fruits,there is a competitive relationship between the synthesis of proanthocyanidins and anthocyanins involving RuANR and RuLAR,and RuMYB8 may negatively regulate the synthesis of flavonoids.The comprehensive research results indicate that the contents of sugars,acids,and flavonoids in blackberry fruits,as well as their interactions and the expression levels of related genes,collectively influence the appearance and morphology,and flavor of fruits.
blackberry(Rubus spp.)fruit qualityphenotypic indexphysiological and biochemical indexcorrelationgene expression
万方数据
维普
