Chromosome identification and karyotype analysis of Eremochloa ophiuroides based on fluorescence in situ hybridization(FISH)technology
Based on the genome sequence data of cultivar'Ganbei'of Eremochloa ophiuroides(Munro)Hack.,a specific single-copy oligonucleotide(oligo)probe library for the chromosomes of E.ophiuroides was excavated and designed through bioinformatics methods,and a series of fluorescence in situ hybridization(FISH)experiments were conducted on the mitosis metaphase chromosomes of root tip cells of E.ophiuroides in combination with telomere repeat sequence and rDNA repeat sequence probes.The results show that the karyotype formula of E.ophiuroides is 2n=2x=18(2SAT),the chromosomes 5 and 4 can be identified by using 5S rDNA and 18S rDNA repetitive sequence probes,and the 18S rDNA enriched region on chromosome 4 is prone to or exhibit breakage phenomena;the specific single-copy oligo probes designed for E.ophiuroides chromosomes can identify chromosomes 2,6,and 9,and by further combining the results of rDNA repeat sequence probes,it is also discovered that a chromosomal translocation has occurred between chromosomes 4 and 8.Based on comprehensive analysis,it is believed that the karyotype of E.ophiuroides exhibits instability,and its genome has undergone chromosomal structural rearrangements during the evolution process.The FISH technology system established in this study based on repeat sequence and single-copy oligo probes can provide a new method for accurate recognition and variation identification of E.ophiuroides chromosomes,and the observed genome karyotype characteristics of E.ophiuroides can provide cytological evidence for the construction of a high-quality genome map of E.ophiuroides.
Eremochloa ophiuroides(Munro)Hack.chromosomerepeat sequenceoligonucleotide(oligo)fluorescence in situ hybridization(FISH)karyotype analysis
万方数据
维普
