Analysis on chemical components and antimicrobial activity of volatile oil from the leaves of Ledum palustre var.dilatatum
The leaf volatile oil of Rhododendron tomentosum Harmaja was prepared by using steam distillation,the chemical components of the leaf volatile oil were separated and identified by using gas chromatography-mass spectrometry(GC-MS)technology,and the inhibitory effects of the leaf volatile oil against eight pathogenic bacteria,one pathogenic fungus,and four plant pathogens was preliminarily explored.The results show that a total of 46 chemical components are identified in leaf volatile oil of L.palustre var.dilatatum,among which,the relative contents of octahydro-2,5-methano-1H-indene and 7-ethyl-1,3,5-cycloheptatriene are relatively high(which are 21.15％and 18.92％,respectively).The leaf volatile oil of L.palustre var.dilatatum exhibits a certain degree of inhibitory effect on eight bacteria and Candida albicans at different mass concentrations,among which,the minimum inhibitory concentration(MIC)against Staphylococcus aureus is the lowest(2 mg·mL-1),and the MIC value against C.albicans is 2 mg·mL-1;the leaf volatile oil of L.palustre var.dilatatum has significant inhibitory effects on four plant pathogens,among which,the median effective concentration(EC50)against Rhizoctonia solani is the lowest(0.19 mg·mL-1).It is suggested that the leaf volatile oil of L.palustre var.dilatatum possesses a broad-spectrum antimicrobial effect,and cuminal,terpinen-4-ol,and α-pinene may be the main components responsible for its antimicrobial activity.
万方数据
维普
