The leaf volatile oil of Rhododendron tomentosum Harmaja was prepared by using steam distillation,the chemical components of the leaf volatile oil were separated and identified by using gas chromatography-mass spectrometry(GC-MS)technology,and the inhibitory effects of the leaf volatile oil against eight pathogenic bacteria,one pathogenic fungus,and four plant pathogens was preliminarily explored.The results show that a total of 46 chemical components are identified in leaf volatile oil of L.palustre var.dilatatum,among which,the relative contents of octahydro-2,5-methano-1H-indene and 7-ethyl-1,3,5-cycloheptatriene are relatively high(which are 21.15％and 18.92％,respectively).The leaf volatile oil of L.palustre var.dilatatum exhibits a certain degree of inhibitory effect on eight bacteria and Candida albicans at different mass concentrations,among which,the minimum inhibitory concentration(MIC)against Staphylococcus aureus is the lowest(2 mg·mL-1),and the MIC value against C.albicans is 2 mg·mL-1;the leaf volatile oil of L.palustre var.dilatatum has significant inhibitory effects on four plant pathogens,among which,the median effective concentration(EC50)against Rhizoctonia solani is the lowest(0.19 mg·mL-1).It is suggested that the leaf volatile oil of L.palustre var.dilatatum possesses a broad-spectrum antimicrobial effect,and cuminal,terpinen-4-ol,and α-pinene may be the main components responsible for its antimicrobial activity.
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