Physiological and Biochemical Characteristics and Root Morphology of Fagopyrum megaspartanium under Hydroponic Culture
In order to study the adaptation ofFagopyrum megaspartanium in hydroponic culture providing theoretical foundation for its hydroponic-culture,a hydroponic experiment was conducted with three different NAA levels (0.27,0.54,1.08mmol/L) to determinate the root growth of F.megaspartanium after 10d and 20d.Total length,surface area and volume of roots were calculated to obtain suitable NAA level for root growth of F.megaspartanium.The branches of F.megaspartanium were treated through the optimal NAA level and put into water to induce adventitious roots.The branches were divided into two groups to be cultured in nutrient solution and soil respectively.The root morphology and physiological indexes were determined after 40d of culture.Results showed that the optimal inducing concentration of NAA promoting F.megaspartanium rooting of water root was 0.27mmol/L;Compared with the short brown hairy roots of the soil cultivated plants,the hydroponic roots were white or light green with secondary roots,but without root hairs.Roots from the hydroponic culture had more fresh weight,root/shoot ratio,total root length,surface area,volume and antioxidant enzymes activities than those from the soil culture.The contents of chlorophyll a,chlorophyll-b,total chlorophyll and carotenoid in hydroponic group were lower than that in soil cultured group except for water content and chlorophyll a/b.The activities of POD,SOD in roots were higher than that in leaves of hydroponic and cultured group (P＜0.05).The activities of POD,SOD in roots of hydroponic group were higher than that in roots of soil cultured group (P＜0.05),and the activity of CAT and the MDA content showed no significant difference.Hydroponic could influence root morphological characteristics,pigment contents and some of the capacity of antioxidant,however part of the morphological,and physiological and biochemical indexes were changed to adapt hydroponic environment.
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NSTL
万方数据
维普
