Multi-Locus Genome-Wide Association Study and Genomic Prediction for General Combining Ability of Maize Ear Diameter
Ear diameter is an important trait for ear in maize,and general combining ability(GCA)is an important index to evaluate parental mating ability in hybrid breeding.A total of 537 F1 hybrid combinations derived from NCII genetic mating design were used as materials.Seven multi-locus genome-wide association study(MGWAS)methods were used to conduct mapping analysis of ear diameter GCA in Xinxiang,Zhoukou,and combined environment,and the effects of different significant loci from MGWAS methods as fixed effects on the prediction accuracy of ear diameter GCA were studied,which will provide a guidance for the breeding utilization of genomic selection(GS)for key loci of ear diameter GCA.The results showed that a total of 18 significant SNPs(P＜8.52E-07)were detected for the GCA effect of ear diameter,of which six loci were simultaneously detected using two to five MGWAS methods and three of them were detected in two environments.Five GS random effect models were used in the three environments,and the prediction accuracy of ear diameter GCA ranged from 0.32 to 0.44.The genomic prediction ability of GCA of ear diameter could be effectively enhanced in all three environments by adding significant loci as fixed effects into the prediction model,increasing the prediction accuracy to 0.38-0.64.
MaizeEar diameterGeneral combining abilityMulti-locus genome-wide association studyGenomic selectionFixed effect model
NETL
NSTL
万方数据
