Transcriptome Analysis Reveals Mechanisms of Calycosin-7-O-β-D-Glucoside Accumulation in Astragalus membranaceus Adventitious Roots through Hydrogen Peroxide
Hydrogen peroxide(H2O2)significantly enhanced the content of calycosin-7-O-β-D-glucoside(CG)in Astragalus membranaceus adventitious roots(AMARs).To discover its biosynthetic mechanism of CG,AMARs were used to screen the differentially expressed genes(DEGs)through transcriptome sequencing after H2O2 treatment.The function and metabolic pathways were analyzed by using up-regulated genes,verified them by qRT-PCR method,and measured the relevant physiological and biochemical parameters.The results showed that AMARs activated ethylene signaling,induced all structural genes in the phenylpropane-isoflavone biosynthesis pathway,significantly increased the content of CG to counteract the oxidative stress caused by H2O2 treatment.Furthermore,H2O2 treatment also induced phenylalanine biosynthesis in order to increase the content of CG.These results revealed the accumulation mechanism of CG in AMARs which laid a theoretical basis for large-scale production of CG in the future.
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